Primary Research Of Vasculogenic Mimicry In Endometrial Carcinnoma

Objective:to explore whether vasculogenic mimicry exists in endometrial carcinnoma which is a tumor special vascular supply model. To explain the clinical significance of this unique vascular supply model,and preliminary investigate the possible molecular mechanism of its occurrence and development.Methods:to collect60cases of endometrial carcinoma resection samples with complete clinical and prognostic data. Double staining with CD34and PAS were conducted to explore if VM exist in endometrial carcinoma and then were used to study the correlation between VM and clinicopathologic factors. Immunohistochemical staining of VEGF and COX-2were conducted to account the proportion of MVD,the staining index (SI) was assessed to compare the different expression between VM-positive group and VM-negative group to explore the role it played in the formation of VM.Results:l.VM channels were detected by using CD34/PAS double staining and HE staining.VM were tumor cells(not endothelial cells) formed channels with PAS-positive materials. There were no CD34-positive cells in VM.13cases have VM of the all60cases(positive rate21.7%).2. The positive rate of VM has no relationships with the age of the patients (P=0.833).3. The rate of VM in poorly differentiated endometrial carcinnoma was significantly higher than that in well differentiated endometrial carcinnoma (P<0.05)4. FIGO stage of the VM group were more advanced than that in non-VM group (P< 0.05)5.The VM group had a higher rate of hematogenous metastases (P<0.05).6. The expression of VEGF,COX-2proteins in poorly differentiated endometrial carcinnoma was significantly higher than that in well differentiated endometrial carcinnoma (P<0.05)7. The expression of VEGF and COX-2proteins were positively correlated in endometrial carcinnoma (P<0.05).8. The expression of VEGF proteins in the VM group was significantly higher than that in the non-VM group (P<0.05)9. The expression of COX-2proteins in the VM group was significantly higher than that in the non-VM group (P<0.05)10. The count of MVD had no difference in statistics between the VM groupand the non-VM group (P>0.05)Conclusion1. Vasculogenic mimicry were exist in endometrial carcinnoma.2. The malignant degree of endometrial carcinnoma cases with VM were higher, and easier to hematogenous matastases.3. The expressive intensity of VEGF and COX-2were positively correlated with pathological grade of the endometrial carcinnoma.as well as the expressive intensity of VEGF and COX-2positively correlated.4. The level of expressive intensity of VEGF and COX-2in the VM group was significantly higher than that in the non-VM group,which suggested VEGF、COX-2may play an important regulating role in the formation of VM in vasculogenic mimicry.