A-FABP And Type2Diabetes,Diabetic Nephropathy Correlation

Objective: Adipocyte fatty acid-binding protein (A-FABP) is a recentlydiscovered human body lipid and glucose metabolism and insulin resistanceplays an important regulatory role in the formation of the protein, and isconsidered an independent risk factor for the development of type2diabetes.The issue of normal glucose and type2diabetes by measuring plasmaA-FABP levels to understand the correlation with type2diabetes, and toinvestigate the relationship between A-FABP and body mass index, bloodglucose, blood lipids, insulin sensitivity and diabetic nephropathy.Method:1. March2013-2013December, the Affiliated Hospital ofNorth Sichuan Medical College for treatment, according to1999WHOregulation,Normal control group(NC)54patients (outpatient physicalexamination).2Diabetes mellitus (T2DM) patients with76cases (AffiliatedHospital ofNorth Sichuan Medical Collegeof Endocrinologyinpatients), whichaccording to urinary albumin/urine creatinine ratio (UACR) be diabeticnephropathy stage:(1) normal albuminuria period (D1): UACR <30mg/gwere26cases;(2) microalbuminuria (D2): UACR≥30mg/g and UACR<300mg/g were32cases;(3) of macroalbuminuria (D3): UACR≥300mg/gin18cases.(2)Using enzyme-linked immunosorbent assay (ELISA) to detect normalglucose regulation in54cases,76patients with type2diabetes fasting plasmaA-FABP levels and get their body mass index (BMI), fasting plasma glucose(FPG), glycated hemoglobin (HbA1C), fasting insulin (FINS), triglyceride(TG), cholesterol (TC), serum creatinine (Scr) and other indicators, andcalculated insulin resistance index (HOMA-IR).3.Using SPSS16.0statistical software to analyze the data before normality test, homogeneity of variancetest, if not met, the number of variables must first be converted to conform tothe normal distribution or homogeneity of variance, mean±standarddeviation (±s)said. Using an independent samples t-test between the twomean, between the average number of multiple samples ANOVA. A-FABPand body mass index (BMI), glucose (FPG), glycated hemoglobin (HbA1C),fasting insulin (FINs), insulin resistance index (HOMA-IR), triglyceride (TG),cholesterol (TC), blood Between the creatinine (Scr).Pearson correlationanalysis and multiple linear regression analysis;using receiver operatingcharacteristic curve (ROC) analysis of A-FABP values for different stages ofdiabetic kidney disease diagnostic performance, calculate the area under thecurve, measured threshold calculate sensitivity and specificity of diagnosis.(3)Results:1.Type2diabetes mellitus (T2DM) in plasma A-FABP levelswere significantly higher than the normal control group, the difference wasstatistically significant (P <0.01).2.Between plasma A-FABP levelcomparison: pairwise comparisons between any two groups were statisticallysignificant differences (P<0.05).3.Pearson correlation analysis showed that:A-FABP and body mass index (BMI), fasting plasma glucose (FPG), glycatedhemoglobin (HbA1C), fasting insulin (FINs), insulin resistance index(HOMA-IR), triglyceride (TG), total cholesterol (TC) were positivelycorrelated (P <0.05); A-FABP and serum creatinine (Scr) no significantcorrelation (P>0.05).4.With type2diabetes mellitus (T2DM) in plasmaA-FABP as the dependent variable, the relevant clinical parameters asindependent variable multiple linear regression analysis showed: HOMA-IR,TG, BMI are significant factors in plasma A-FABP’s (P<0.05).5.Obtained bythe ROC curve: plasma A-FABP in NC and D1distinguish the area under the curve of0.767， plasma A-FABP in D1and D2distinguish the area under thecurve of0.859, plasma A-FABP in D2and D3distinguish the area under thecurve of0.794.Conclusion:1. A plasma A-FABP in patients with type2diabetes and indifferent higher than normal diabetic kidney disease staging, A-FABPdifferences were statistically significant.2.Plasma A-FABP and body massindex (BMI), fasting plasma glucose (FPG), glycated hemoglobin (HbA1C),fasting insulin (FINs), insulin resistance index (HOMA-IR), triglyceride (TG),cholesterol (TC) were positively correlated. Which body mass index (BMI),triglyceride (TG), insulin resistance index (HOMA-IR) is significantlyinfluencing factors of plasma A-FABP.3.Plasma A-FABP levels can beconsider for the staging of diabetic nephropathy as a reference indicator.