| Object: With the development of economy and the transformation ofconcept, beauty demand has been increasing year by year in the world, and avarious of beauty technology has been appeared. The plasma skin regenerationsystem is a novel device to rejuvenate skin that utilizes the energy of plasmaby creating thermal effect, which has been widely employed in fine lines,yspigmetation, light aging, skin relaxation, scar and other skin lesions.The plasma is emitted in a millisecond pulse to deliver energy to targettissue upon contact without reliance on skin chromophores, and causinginstantaneous heating without an explosive effect on tissue or epidermalremoval. Histologically, immediately after PSR treatment, an intact andnonablated epidermis with vacuolation of the basal cell layer is visible, whichis the zone of thermal damage. And the zone of thermal modification under thezone of thermal damage, whose cells is still alive but modification. Thermaldenaturation of collagen fibers cause immediate tissue contraction, the thermaldamage of elastic fiber and activation of fibroblasts promote wound healing,which lead to new collagen and lightened solar elastosis.Although PSR has been very effective, a majority of patients can’t standthe accompany pain. For that, anesthesia is closely related to the PSR. Inrecent years, topical anesthesia is the main method to assist PSR while itsshortcomings are unavoidable (see below):1A long waiting time: This is often so slow that the patients have to waita significant amount for time about1-2hours to achieve a better result.2Skin hydrated: A further Pre-clinical study was proved that hydration ofthe epidermis defines the amount of energy that is absorbed.3Unsatisfied anesthetic effect: Potter’s research shows that the averagepain score of patients is4,which is according to the parameter linear simulation scores that is on a scales of1to10. So many people can’t achieve asatisfied anesthesia effect.4Complications: contact dermatitis is common.In conclusion, topical anesthesia is not perfect. So we raise acomplementary method that we apply the subcutaneous infiltration anesthesiain the PSR. According to it, we study aim at evaluating the effects of PSR afterusing topical anesthetization as compared to subcutaneous infiltrationanesthesia, thereby inquiring into the advantages and disadvantages ofsubcutaneous infiltration anesthesia and increasing electivity for clinical work.The two anesthetizations were compared for the skin necrosis degree and thenew collagen fiber thickness.Methods:14SD rats were randomly utilized for this controlledexperiment. All rats were given intraperitoneal injection of chloral hydrate,dose of0.3ml/100g.After adequately sedated, the rat was stuck in a board andits hairs on the side of back was removed by using electric razor. Followingdepilation,the rat’s back was randomly divided into three experimentalregions that was marked as A、B、C. To recognise the treated area, my red gelpen gives circles whose diameters are all1cm on every region. The circles inA were treated by external application of5%compound lidocaine cream for1hour,whose thickness was1-2mm,and the cream was wiped off by using drycotton stick2minutes before PSR. The circles in B were treated by injectingthe2%lidocaine hydrochloride injection with a dose of0.2ml intosubcutaneous tissue, then every circle uplifted uniformly, and then given PSRtreatment. The circles in C were treated solely by PSR without any anesthesia.Every circle was achieved fixed point and single treatment by using energysetting of120W, and the frequency was1.0s.Biopsies were taken from alltreatment sites of seven rats randomly in pre-operation and4days followingtreatment for a total of14groups, and other rats in30days followingtreatment for a total of14groups. All biopsies are full-thickness skin tissueand the diameters of them are2mm.Biopsies in4days were processed tohematoxylin-eosin(HE) staining in order to observe the skin necrosis degree, and in30days were processed to Van Gieson (VG) staining respectively inorder to observe the new collagen fiber thickness, and in pre-operation wereprocessed to HE staining in order to compare with others. Histopathologicexamination was performed by observers blinded as to the treatmentconditions. Differences in skin necrosis thickness and new collagen fiberthickness among the study groups were tested by One-Way ANOVA. Whenthe overall difference was significant in analysis of variance, an intergroupcomparison was performed by Student-Newman-Keuls test. The differencewas considered significant at P＜0.05. All data are expressed as Mean andStand Deviation(±S).Results: the result of the skin necrosis degree is143.29±40.90μm in Aregion,311.56±40.79μm in B region and220.58±34.76μm in C group,respectively. In this analysis, there was a statistically significantreduction(P<0.01) in the skin necrosis degree of topical anesthesia groupcompared to the other two groups, while a statistically significant rise(P<0.01)in the subcutaneous infiltration anesthesia group. The results of the newcollagen fiber thickness are135.38±29.88μm in A region,540.53±51.84μm inB region and368.61±55.75μm in C region, respectively. According to thatdata, there was a statistically significant reduction (P<0.01) in the newcollagen fiber thickness of topical anesthesia group compared to the other twogroups, while a statistically significant rise (P<0.01) in the subcutaneousinfiltration anesthesia group. These results suggests that skin was appliedsubcutaneous infiltration anesthesia showed a deeper range of tissue effectsvia the treatment of the plasma skin regeneration system as compared to thetopical anesthesia and the control group, Meanwhile, the effect of the topicalanesthesia was the lowest.Conclusion: The effects of PSR treatment on the skin are potentiated bysubcutaneous infiltration anesthesia，while the topical anesthesia is the exactopposite. So the subcutaneous infiltration anesthesia for PSR treatment wassuperior to the topical anesthesia. |
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