Study On The Effect Of KiSS-1on The Biological Behavior Of Human Trophoblast Cell And Mechanisms In Recurrent Spontaneous Abortion

AIM: To explore the effect of KiSS-1on the biological behavior of humantrophoblast cell and mechanisms in recurrent spontaneous abortion.METHODS:①Compared the expression of kisspeptin and GPR54in maternal-fetalinterface of recurrent spontaneous abortion and normal pregnancy byimmunohistochemistry;②The recombinant plasmid pcDNA3.1（+）-KiSS-1wasconstructed successfully;③The recombinant plasmid pcDNA3.1（+）-KiSS-1wastransfected into human trophoblastic tumor cell lines JAR and establish monoclonal celllines;④Detect KiSS-1mRNA and encoded peptide expression in transfected cells byRT-PCR and Western Blot;⑤Detect the effect of KiSS-1gene and kisspeptin on JAR cellproliferation, ability of colony formation in soft agar, migration and invasion abilities.RESULTS：①The expression of ksspeptin/GPR54in villi of RSA group weresignificantly lower than the control group（P<0.05）. Kisspeptin expression in decidua wassignificantly lower in RSA group than the control（P<0.05）. There was no difference ofGRP54expression in decidua between the RSA group and the control;②Eukaryoticexpression vector pcDNA3.1（+）-KiSS-1was successfully constructed and identified byPCR and sequencing;③The recombinant plasmid pcDNA3.1（+）-KiSS-1successfullytransfected into JAR cell lines;④Selected stably transfected cells and positive expressionof KiSS-1mRNA and encoded peptide kisspeptin were identified by RT-PCR and WesternBlot;⑤KiSS-1gene and kisspeptin had no effect on proliferation and colony formingability of JAR cells, but significantly inhibited its migration and invasion.CONCLUSION: The expression of kisspeptin in villi and decidua of women withrecurrent spontaneous abortion was significantly lower than the control group, GPR54 expression in villi of women with recurrent spontaneous abortion was significantly lowerthan the control group.KiSS-1gene and its production kisspeptin significantly inhibitsmigration and invasion of trophoblast cells. We suggest that the expression and regulation ofKiSS-1in the maternal-fetal surface play an important role in the maintenance of normalpregnancy, and its abnormal expression may be associated with recurrent spontaneousabortion.