Expression Of TRF1and TRF2mRNA And Protein In The Peripheral Blood Mononuclear Cells Of Patients With Primary Gouty Arthritis

Objective: To investigate the expression levels of telomeric proteinTRF1and TRF2mRNA and protein in the peripheral blood mononuclear cells(PBMCs) of patients with primary Gouty arthritis(GA), and the relationsbetween these gene expression levels and disease activity are explored.Methods:(1)126GA [including77Acute Gouty arthritis（AGA）and49non-acute gouty arthritis (NAGA) patients] and79healthy controls (HC)were enrolled into the study. The clinical data of primary gouty arthritispatients and healthy controls were collected.PBMCs were isolated from bloodin vitro.(2) Real-time quantitative polymerase chain reaction(RT-qPCR) wasemployed to study the expression of TRF1and TRF2mRNA in peripheralblood mononuclear cells(PBMCs)from77AGA,49NAGA and79HC,and thedifferences of gene expression in three groups were compared.(3) TRF1andTRF2portein were measured by using Western Blot (WB) in PBMCs from15GA,9NAGA and12HC,and the otherness of protein expression in threegroups were compared.(4) Spearman’s correlation and multiple regressionanalysis or Wilcoxon test were used to evaluated relationship among mRNAlevel and the index which were detected.Analysis of covariance or Wilcoxontest and Spearman’s correlations were used for statistical analysis.Results: The expression levels of TRF1and TRF2mRNA in the PBMCsof AGA and NAGA patients significantly increased compared to healthyindividuals (P<0.01).The expression levels of TRF2was correlated with LY(r=0.289, P<0.05). The expression levels of TRF1was correlated withTRF2(r=0.935, P<0.01)、LY (r=0.256, P<0.05)、TG (r=0.281, P<0.05)、VLDLC (r=0.291, P<0.05).Conclusion: TRF1and TRF2play a key role in the inflammatoryimmune regulation of GA, and they are involved in the pathogenesis of goutyImmune and inflammatory responses. Further study would be needed inexpression and functions of telomeric proteins.