Expression And Significance Of PEG10in Placenta Of Patients With Severe Preeclampsia

Objective: Hypertensive disorder complicating pregnancy(HDCP),whichincludes gestational hypertension, preeclampsia,eclampsia,preeclampsiasuperimposed upon chronic hypertension and chronic hypertensioncomplicating pregnancy,is the most common complication of obstetric.Preeclampsia is unique to pregnancy disease， preeclampsia is a systemicdisease，which appears after20weeks of gestation with hypertension(≥140/90mmHg), proteinuria (≥300mg/24h)and may be accompanied byepigastrium discomfort, headache, blurred vision, etc. And when the bloodpressure in preeclampsia patients rose to160/110mmHg, or urine proteinreaches2.0g/24h, severe preeclampsia appears. At home and abroad, theincidence of sPE is high, which seriously threatens the maternal and childhealth. The etiology of sPE has not been fully elucidated, At present, a lot offoreign scholars think the disease can be divided into two stages: the badplacenta formation and placenta oxidative stress. Placentation is poor, mainlyfor the bad villi sertoli cell erosion. completely Erosion of nourish cellscause abnormal uterine spiral arteriolar "vascular remodeling",and the bloodflow to the placenta reduces, ischemia and anoxia promote placenta releasingfactor, lead to a wide range of system inflammatory reaction. Imprinted geneis a kind of single allele and only expresses male parent or female parentaccording to the different source. Imprinted gene plays an important role to thegrowth of early mammalian embryo, fetus and placenta. Normally, thepaternal expressed gene(maternal imprinted) imprinted genes promote thenutrition intake and embryonic development, and maternal expressed (paternalimprinted) imprinted genes reduce and weaken it. paternally expressed gene10(PEG10) is one of the paternal expressed gene(maternal imprinted)imprinted genes,which expressed in ovary, brain, placenta, fetal liver tissue of adult. The gene plays an important role in the early differentiation of fat cellsand the occurrence, development and metastasis of many kind of tumors hasclose relationship, as well as in the placenta formation, trophoblast invasionand differentiation. According to the relationship of PEG10with invasion andmetastasis of cells, We speculate that its abnormal expression may changeinfiltration of sertoli cell, and in the occurrence and PEG10plays an importantrole in development of severe preeclampsia. Our main purpose is exploringthe role of PEG10in the onset of severe preeclampsia through the detection ofPEG10in placenta tissue of severe preeclampsia patients, for looking for theetiology and pathogenesis of severe preeclampsia.Materials and Methods：Specimens selected from March2013toOctober2013in the maternity of The Second Hospital of He Bei MedicalUniversity.The normal pregnant women and the patients with severepreeclampsia were120cases. Select28-34weeks of pregnancy in patientswith severe preeclampsia30cases as early onset group, a the same periodnormal pregnancy30cases as early control group（Induced labor as fetalabnormalities），34-42weeks of pregnancy in patients with severepreeclampsia30cases as late onset group, a normal pregnancy30cases as latecontrol group. The diagnosis of severe preeclampsia according to the people’smedical publishing house, Jie Le editor of journal of 《obstetrics andgynecology》(7th edition) standard, the pregnant women were selected as thefirst child, the ages of pregnant women were22-29, there was no statisticallydifference, without the history of essential hypertension, diabetes, heartdisease and kidney disease, thyroid dysfunction, etc. Samples were drawnfrom maternal placenta placenta villi surface near the umbilical cord roottissue, about1.0㎝×1.0㎝×1.0㎝,and keep away from the hemorrhage,necrosis and calcification area. Fixed with4%formaldehyde solution,embeded paraffin,and prepared paraffin sections. And then tested PEG10expression in placenta tissues with immunohistochemical method.All of the experimental data used SPSS17.0statistical software for dataprocessing, differences between the two groups with rank sum test. The inspection standard was α=0.05, P<0.05as a statistically significantdifference.Results:1PEG10proteins are expressed in all the groups, mainly insyncytiotrophoblast and cytotrophoblast, and the quantity insyncytiotrophoblast is higher than cytotrophoblast. PEG10proteins express inthe cell membrane and cytoplasm and can not be seen in nucleus.2The early onset group PEG10protein expression in placenta tissue isobviously higher than early control group, and the difference was statisticallysignificant(P<0.01).3The late onset group PEG10protein expression in placenta tissue isobviously higher than late control group, and the difference was statisticallysignificant(P<0.01).4The early onset group PEG10protein expression in placenta tissue ishigher than late onset group, and the difference was not statisticallysignificant(P>0.05).Conclusions: Both early onset group and late onset group proteinexpression in placenta tissue is higher than control groups,which prompts thatthe Higher expression of PEG10protein in placenta tissues may be associatedwith the occurrence of severe preeclampsia， PEG10may affect themigration,invasion and other functions of sertoli cell, leads to shallow placentaimplantation,cause severe preeclampsia. the difference between early onsetgroup and late onset group was not statistically significant.