Study Of The Relationship Between Leptin, Leptin Receptor And Invasion And Metastasis In Female Breast Cancer

Objective: The incidence of breast cancer increases year by year,2013data show that20-59year-old breast cancer to become the main reason forfemale cancer deaths. The incidence of breast cancer is associated withmultiple endocrine factors, such as insulin, estrogen and leptin. Leptin ismainly synthesized and secreted by fat cells. Leptin contains167amino acids,production of obese (Ob) gene, leptin can promote cancer incidence,progression, invasion, metastasis and recurrence. The relationship betweenobesity and cancer has become the social epidemiology of health problems.WHO reported that there are at least400million people are obese now, whilethere will be over700million obese persons in2015. This study was tocompare the BMI (Body Mass Index, BMI) in breast cancer patients andpatients with benign breast diseases and the differences between the fivegroups of leptin and leptin receptor expression, and the expression of leptin inserum. The study was to explore the relationship between leptin and leptinreceptor expression and breast cancer invasion and metastasis.Methods:All patients were collected form the Fourth Hospital of HebeiMedical University Breast Center Since February2013to June2013.including98patients with benign breast disease,97with breast cancer.Measured of benign breast disease and breast cancer patients’ height, weightand calculated BMI of the two groups. All patients were women. The tissue ofmetastasis with breast cancer is obtained by needle biopsy. All benign andmalignant disease were dignosed by pathology. SP immunohistochemicalmethod was detected expression of the leptin and leptin receptor in tissue.ELASE was detected expression of serum leptin. Application of SPSS13.0statistical software for two sample size and diversity of the amount of the ranksum test, with a=0.05level for the test. Results:1All195subjects,98patients with benign breast disease,97with breastcancer. Two samples of benign breast cancer patients with no significantdifference in age (P>0.05), the age of benign breast disease is43.9±6.9years,while breast breast cancer patients is45.5±6.0years, BMI of benign breastdisease is24.36±3.74, BMI is25.77±4.12with breast cancer. Breast cancerpatients have a higher BMI than patients of benign breast disease, with asignificant difference (P=0.028). Relative risk(RR) is1.441.2The female breast cancer (FMC) patients in this study were divided intofive groups, namely Group A: no axillary lymph node metastasis,9cases;Group B:1-3axillary lymph nodes metastasis, seven cases; C group:4-9axillary lymph nodes metastasis,7cases; D group: more than9axillarylymph nodes metastasis,7cases; E group: distant metastasis,5cases. Leptinin different tissues grouped sequentially increased. Expression of leptin in thetissue of five groups A:163.87±16.33, B:147.24±8.03, C:133.54±18.82, D:105.73±10.39, E:81.52±8.72. The comparison between groups A and B, P=0.012; A and C, P=0.000; A and D, P=0.000; A and E, P=0.000; B and C,P=0.188; B and D, P=0.000; B and E, P=0.000; C and D, P=0.004; C andE, P=0.000; D and E, P=0.049.3Leptin receptors in different tissues grouped is sequentially increased.Expression of leptin receptors in tissues of five groups: A:147.93±12.31, B:125.03±16.15, C:113.85±17.59, D:107.25±12.85, E:80.47±7.38. Thecomparison between groups A and B, P=0.011; A and C, P=0.000; A and D,P=0.000; A and E, P=0.000; B and C, P=0.083; B and D, P=0.014; B andE, P=0.000; C and D, P=0.004; C and E, P=0.000; D and E, P=0.012.4Serum leptin in different grouped is sequentially increased. Expressionof leptin in the tissue of five groups A:16.378±0.471, B:21.393±1.551, C:28.978±2.570, D:35.706±2.942, E:39.186±7.377. The comparison betweengroups A and B, P=0.000; A and C, P=0.000; A and D, P=0.000; A and E,P=0.000; B and C, P=0.000; B and D, P=0.000; B and E, P=0.000; C andD, P=0.000; C and E, P=0.000; D and E, P=0.329. Conclusion:1The breast cancer patients’ BMI is higher than in patients with benignbreast disease, suggest that obesity is a risk factor for breast cancer.2Leptin and leptin receptors in five tissues grouped is sequentiallyincreased, Indicating leptin and leptin receptors promote the metastasis andrecurrence of breast cancer.3Leptin in five serum grouped is sequentially increased, Indicating leptinpromote the metastasis and recurrence of breast cancer.