| Objective: Liver transplantation as a method to save patients withend-stage liver disease has been known and accepted by more and morepeople,liver transplant surgeon is mature technology, and with theimprovement of economic development level, gradually become a routineprocedure, although the use of immune inhibitor play a key role in thetransplantation immunity, but the graft rejection is still the most important inliver transplantation patients after death.Th17cells were found in recent years a specific effective secreteinterleukin17(interleukin17, IL-17) of helper T cells, biological functionsand different from that of Th1and Th2cells.In our country focus on the studyof Th17cells in autoimmune diseases,cancer,animals,transplantation immunity,etc,and the human organ transplant research report, affect the influence factorsof patients after orthotopic liver transplantation is very complex.The purpose of this paper is to research on chronic hepatitis b cirrhosispatients after orthotopic liver transplantation the change of the content of Th17cells in peripheral blood, to clarify after orthotopic liver transplantationpatients Th17cells changes the level of clinical significance.Methods: Collection of15patients with orthotopic liver transplantation(olt) for the first time before surgery, postoperative7days,14days,21days ofperipheral blood, will appear in patients with acute rejection compared withpatients who were not appear acute rejection, the application of automaticbiochemical analyzer test in patients with various points in the liver andbiochemical indicators and FK506concentration, flow cytometry instrumentdetection of T cells and Th17cells, all the patients in group were routinepathological examination in order to make clear whether acute rejection, once considered in patients with acute rejection, do pathological examinationshould be repeated in order to make clear diagnosis, comparison of Th17cellschanges between patients and normal controls, Th17cells in patients with thechange of each point in time, and associated with liver biochemical indicators,FK506concentration to do analysis.Results：1. The experiment successfully collected altogether15patientswith orthotopic liver transplantation, of which3cases of acute rejection,examined the statistical data from the two groups the same overall comparable(table1).2. Hepatitis b cirrhosis of the liver does not appear after orthotopicliver transplantation in patients with acute rejection of frequency (n=12) Th17cells:7days (3.23+0.63)%,(2.97+0.54)%for14days postoperatively, after21days (1.77+0.81)%, showed a trend of gradual decline, the preoperativelower (P <0.05), and the occurrence of acute rejection reaction of the patients(n=3) frequency Th17cells, after7days to14days (4.76+0.32)%,postoperative (5.41+0.46)%,(6.32+0.71)%for21days postoperatively,significantly higher than that in patients with acute rejection.(3) in patientswith acute rejection, various periods of ALT, AST, DBIL were higher than notpresent acute rejection patients.4, in patients with acute rejection and notpresent day14patients with acute rejection of FK506concentration wasstatistically difference (P<0.05) in5, acute rejection patients and the acuterejection does not appear to detect T cell subgroup of patients have nostatistical difference (P>0.05).Conclusions:1.Chronic hepatitis B patients with cirrhosis does notappear after orthotopic liver transplantation patients with acute rejection ofTh17cells compared with preoperative reduced, in patients with acuterejection of Th17cells compared with preoperative rise.2. Chronic hepatitis bcirrhosis of the liver, after orthotopic liver transplantation in patients withacute rejection of peripheral blood Th17cell levels are not present in patientswith acute rejection of rise.(3) in patients with acute rejection may be relatedto postoperative patients with relatively low concentration of FK506related4.Th17cells may be a detection of acute rejection after liver transplantation patients a detection index. |
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