| Objective: With the increase of obesity and diabetes, the incidence ofdiabetic fatty liver are significantly increased. If not get effective treatment, itcan further develop for hepatitis, cirrhosis, and even liver cancer. Diabeticfatty liver disease is liver lipid metabolism disorders caused by a lack ofinsulin secretion. Obesity with insulin resistance(IR) is common mechanismof type2diabetes and fatty liver. IR refers to that the sensitivity of theorganization response to insulin is decreased. IR plays an important role in thepathogenesis of type2diabetes. Normally, insulin keeps plasma glucose in thenormal range by inhibiting the output of hepatic glucose and by promotingglucose into muscle tissue. IR can not effectively inhibit the output of hepaticglucose and promote glucose into muscle tissue, thus it results in type2diabetes. IR also plays an important role in the pathogenesis of fatty liver.Onthe one hand, IR promote the decomposition of fat for free fatty acids, freefatty acids enter into liver with blood, then it compound fat in the liver cells.On the other hand, IR can inhibit catabolism of fat in liver cell, causing fataccumulation in liver cells. So improving IR has certain therapeutic effect ofdiabetic fatty liver. In recent years, study found that suppressors of cytokinesignaling protein-3(SOCS-3) plays a very important role in IR and thepathogenesis of nonalcoholic fatty liver disease. On the other hand, SOCS-3can lead to IR by inhibiting transduction of insulin signal. On the other hand,it can promote the synthesis of fatty acids by raising Sterol regulatoryelement binding protein-1c(SREBP-1c),resulting in the deposition of lipidin liver, and then leading to the fatty liver. Therefore diabetic fatty liver can beeffectively treated by reducing the expression of the SOCS-3and SREBP-1 c. This experiment was to investigate the effect of promoting blood circulationto remove blood stasis traditional Chinese medicine on suppressors ofcytokine signaling protein-3(SOCS-3) and Sterol regulatory element bindingprotein-1c (SREBP-1c) in diabetic fatty liver rats and to explore potentialmechanisms involved.Methods: Fifty healthy male Sprague-Dawley rats were randomLydivided into five groups: normol group, model group, Chinese medicine group1(salvia miltiorrhiza, rhizoma ligustrci wallichii); Chinese medicine group2(leech, lumbricus); Chinese medicine group3(salvia miltiorrhiza, rhizomaligustrci wallichii, leech, lumbricus). Diabetes was induced withstreptozocin(50mg/kg i.p.), except for normol group. And the latter threegroups were treated with the Chinese traditional medicine which promoteblood circulate and remove the stasis intragastrically once a day for twelveweeks. Triglyceride （TG） and Cholesterol （TC） were measured bybiochemical methods. The expression level of SOCS-3and SREBP-1cmRNAwere determined by RT-PCR.Results:1General condition of experimental rats:Rats of normal group were well,normal size, sensitive reaction, and their weight were increased. The rats in theother groups were mainourished, emaciated, unresponsive and grumpy.2Pathological changes of liver tissue in each group: Compared to modelgroup, the steatosis of liverwere significantly reduced in each drugintervention group; however, the steatosis of liverwere was significantlyseriouser in Chinese medicine group1and2than in Chinese medicine group.3Comparion of TG and TC in different groups: Compared to thenormal group,the expression of TG and TC were increased in model groupobviously（P<0.05); however the expression of TG and TC were significandlylower in each drug intervention group than in model group（P<0.05); theexpression of TG and TC were significandly higher in Chinese medicinegroup1and2than in Chinese medicine group3(P<0.05); there was no significant change in the expression of TG and TC between Chinese medicinegroup1and Chinese medicine group2（P>0.05）4Comparion of ALT and AST in different groups: Compared to thenormal group, the expression of ALT and AST were increased in model groupobviously （P<0.05); however the expression of ALT and AST weresignificandly lower in each drug intervention group than in model group（P<0.05); the expression of ALT and AST were significandly higher inChinese medicine group1and2than in Chinese medicine group3(P<0.05);there was no significant change in the expression of ALT and AST betweenChinese medicine group1and Chinese medicine group2（P>0.05）5The expression levels of SOCS-3and SREBP-1c mRNA in differentgroups: Compared to the normal group, the expression of SOCS-3andSREBP-1c mRNA wer increased in model group obviously(P<0.05); however,the expression of SOCS-3and SREBP-1c mRNA were significandly lower ineach drug intervention group than in model group (P<0.05; Compared toChinese medicine group3, the expression of SOCS-3and SREBP-1cmRNAwere higher than that in Chinese medicine group1and2(P<0.05); Comparedto Chinese medicine group2, the expression of SOCS-3and SREBP-1cmRNAwere higher than that in Chinese medicine group1(P<0.05)Conclusions:1Combined prescription and separated prescription of promoting bloodcirculation to remove blood stasis traditional Chinese medicine can improvefatty degeneration and liver function obviously, having a good effec of thetreatment of diabetic fatty liver.2Combined prescription and separated prescription of promoting bloodcirculation to remove blood stasis traditional Chinese medicine can alleviatefatty liver through decreasing the expression of SOCS-3and SREBP-1cmRNA. Combined prescription was better than separated prescription inalleviating fatty liver.3The Chinese traditional medicine which can promote blood circulateand the Chinese traditional medicine which can dredge collaterals can promote each other in the treatment of diabetic fatty liver. |
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