The Researehes On Protection Of Glomerular Filtration Barrier By Fangji Huangqi Decoction

Objective:Through the observation of the expression of Fangji Huangqi Decoction effects on the Adriamycin Induced Nephropathy (AIN) rats, try to discuss the mechanism for treating glomerulopathy.Methods:48Wistar rats,8of them were randomly taken as normal control group, the others received intravenous injections of ADR via tail vein (the first dose was5.5mg/kg, the second injection dose was3mg/kg after14days), established adriamycin-induced nephropathy rat model, the model rats were randomly divided into model group, treating group by high-dosage and ow-dosage medicine, and then administered intragastrically by medicine in corresponding dosage. In next5weeks, rats in each group were detected24hours urinary Protein. Renal tissues routine was fixed, histopathology change of kidney was observed by electron microscope, CD2-associated protein in renal tissue was detected using immunohistochemistry, and HPA expression was detected using reverse transcription PCR.Results:24h urinary protein quantitative and Heparanases expression of the rats in the model group increased compared with the normal group, the above indexes in treating group by high-dosage, and low-dosage medicine were improved in different degrees.Conclusion:1. Fangji Huangqi Decoction can reduce the quantity of24hours urine protein of Adriamycin Induced Nephropathy rats, alleviate the histopathology change of kidney, protect the charge barrier against damage2. One of the effective mechanism of Fangji Huangqi Decoction is repairing glomerular filtration barrier damage by increasing CD2-associated protein expression and decreasing heparanases expression.