The Association Between Polymorphisms In Il-18and The Unexplained Recurrent Spontaneous Abortion

ObjectiveTo investigate the association among polymorphisms (the promoter rs187238, the exon rs360718, the exon rs360717) in IL-18and the risk of unexplained recurrent spontaneous abortion (URSA)MethodPolymerase chain reaction in combination with DNA sequencing were used to determine the polymorphism of rs187238, rs360718and rs360717respectively,in207patients with URSA and144women with healthy pregnancy.ResultThere was significant difference of rsl87238polymorphism between the URSA group and the control group on genotype frequency or allele frequency (22.7%VS4.2%, X2=22.767, P=0.000;13.04%VS2.1%, x2=26.102, P=0.000). The relative risk analysis about allele frequency were found that C allele carriers of the risk of spontaneous abortion was7.050times of G allele carriers(OR=7.050, CI:2.990-16.622). There was no significant difference of rs360718å’Œrs360717polymorphism between the URSA group and the control group on genotype frequency (X2=1.497, P=0.221;X2=0.858, P=0.354; respectively). Besides, there was also no significant difference of rs360718å’Œrs360717polymorphism between the two groups on allele frequency (P>0.05).ConclusionsThe present study suggested that the rs187238in IL-18gene loci GC+CC genotype may be recurrent miscarriage susceptible genotype, C allele may be one of the genetic susceptibility genes of the disease. Besides, The present study suggested that these polymorphisms(rs360718and rs360717)in IL-18exon may not be associated with URSA.