Effect Of Various Oligodeoxynucieotide On The Proliferation Activity Of Osteoblast Invaded By Porphyromonas Gingivalis

Chronic periodontitis is the inflammatory disease occurring in theperiodontal support tissue. Dental plaque biofilm is the initiating factor ofthe disease. Bacteria and their toxic products can destruct periodontaltissue directly and induce the early inflammatory response with theincrease in number of pathogenic bacteria.However, more recent datashow that the host immune response to pathogens inappropriately is themain cause of periodontal tissue destruction.As an important periodontalpathogen, Porphyromonas gingivalis’ various virulence factors can causeperiodontal tissue destruction by acting directly or indirectly causing hostimmune inflammatory response.Oligodeoxynucleotide is a general term for a class of short chainDNA molecules composed of dozens of nucleotide monomers.ODNhaving immune regulating function can inhibit the excessive immuneresponse caused by infection of the pathogenic microorganisms, so as tomaintain the immune balance state of the body. ODN is easy tosynthesize and can be modified.It has the biological characteristics ofstable structure and property,high efficience,low toxicity and which can enter the cells on their own to play a role without building any carrier.Researches have shown that the ODN is safe and reliable.ODN has beenwidely used in clinical research indicating good application prospects.The overall goal of treatment of chronic periodontitis is not only tocontrol the bacterial infection and eliminate inflammation, but also toinhibit host immune inflammation response caused by bacteria and theirmetabolites generated in periodontal tissue destruction, so as to promoteperiodontal tissue repair and regeneration of different levels and restorethe physical shape and functions of periodontal tissue.The osteoblastproliferation plays an important role in the reparation and regeneration ofthe alveolar bone.ODN has the species specificity and individualdifferences, so this study is to screen out the ODN from differentsequences,which has the effect on the proliferation activity of osteoblastinvaded by Porphyromonas gingivalis,in order to establish the experimentbasis for applying the ODN to regulate periodontal immune inflammationresponse and to promote the reparation and regeneration of periodontaltissue.Methods: ODN used in the experiments were designed bydepartment of molecular biology，college of basic medical sciences,JilinUniversity.ODN were synthesized by Dalian TaKaRa BiotechCompany.We chose P.gingivalis mode strain ATCC33277as experimental strains which were cultured in conventional anaerobic condition. Wechose human osteoblast like cell line MG63cells as experimental cellsand established the model of P.gingivalis internalizing MG63cells.weused the method of MTT to detect the effect of ODN on the proliferationactivity of MG63cells infected by P.gingivalis for2h,4h,8h and12h,then we screened out the ODN having the effect on the proliferationactivity of MG63cells for further study that used the method of MTT todetect the effect of ODN on the proliferation activity of MG63cellsinternalized by P.gingivalis for2h,4h,24h and48h.Results: Compared with the PBS control group, ODN BW001,FC003, FC004, SAT05f and MT01have the effect on promotingproliferation of MG63cells infected by P.gingivalis for2h,4h,8h and12h.The results are statistically significant (P <0.05, P <0.01).Comparedwith the PBS control group, ODN FC003has the effect on promotingproliferation of MG63cells internalized by P.gingivalis for2h,4h,24hand48h.The results are statistically significant (P <0.05).Conclusion: ODN BW001, FC003, FC004, SAT05f and MT01canpromote the proliferation of MG63cells infected by P.gingivalis.ODNFC003can also promote the proliferation of MG63cells internalized byP.gingivalis.