The Influence Of Lentiviral-vector Silencing COX-2Gene On The Drug Sensitivity Of Tca8113Cells Of Tongue Squamous Cell Carcinoma

Tongue squamous cell carcinoma (TSCC) is deemed as one of the most common oral malignancy.In our country, its incidence among the oral cancer is the highest, occupyingy approximately39.95%. The researchers have not yet found out its pathogenes and they hold that the incidence of it concerns a variety of factors. In recent years, the incidence of the disease is increasing considerably and more and more young people get infected with this disease. Tongue squamous cell carcinoma grows rapidly and its degree of malignancy is high. It also gets the characteristic of strong invasion. The surrounding mucosa and tongue muscles can be easily infected by the tongue squamous cell carcinoma and the tongue squamous cell carcinoma can results in limited mobility as well as a number of tongue dysfunction.In the prostaglandin(PGs) biosynthetic process, Cyclooxygenase (COX) plays the role as a very important rate-limiting enzyme. There exist two known COX isoforms. Several studies show that COX-2has an over-expression in various tumor tissues.RNA interference (RNAi) is one of the methodologies to induce the expression of some specific Gene silencing. A large number of studies showed that the great potential of this technique in reverse gene analysis and gene therapy, and its high efficiency, specificity and stability is also impressive.By lentivirus mediation, we got the expression of COX-2in tongue squamous carcinoma cell lines Tca8113cells silenced. Following COX-2knock-down, the confirmation of the expression of COX-2was done by western-blot. The groups consist of COX-2gene silencing group (pLKO.1shCOX-2), negative control group (scr) and untreated cell group. The drug sensitivity of COX-2altered cells were analyzed by MTT.The results of this study showed that Tca8113cell lines with COX-2gene silencing were established successfully. The inhibition rate of pinyangmycin and cisplatin and with different concentrations on three groups of cells were detected by MTT after48h. The result demonstrated that the suppression rate of negative control group (scr) and untreated cell group is lower than different concentrations of drugs on cells of COX-2gene silencing group (pLKO.1shCOX-2). Statistically, the results were important.Our studies demonstrated that knock-down COX-2enhanced Tca8113cell line cells’sensitivity to pinyangmycin and cisplatin and suppressed the tumorigenicity of them. The conclusion we get in this thesis shows that there exists a correlation between COX-2and cellular growth.