The Study For Fabrication,Characterization And Biocompatibility Of Non-linear Biodegradable Poly(glycerol-sebacate) Elastomer

Purpose: This study evaluated the utilization potentiality in soft tissue engineering field of PGS(poly glycerol-sebacate) according to the material’s fabrication, characterization of physical and chemical property and measuring of biocompatibility.Materials and Methods:PGS in this assay was synthesized from glycerol and sebacic acid with1/1,1/2mole ratio by the method of melting condensation. The molecular structure, mass and crystalline degree of PGS were characterized by fourier transform infrared spectroscopy(FT-IR), gel chromatography(GPC) and X-ray diffracation analysis(XRD). The properties of mechanics, hydrophily and degradability were measured by tensile test, water contact angle analyzer as well as degradation test. Electrospun films were produced from PGS and PLLA, and characterized by scanning electron microscopy (SEM) and water contact angle analyzer. Rabbit BMSCs were isolated and purified in vitro, to seed the Rabbit BMSCs of3rd-generation onto the electrospun films. To analyze the compound degree in vitro by scanning electron microscopy (SEM) on5th,10th,15th day after seeded, then to HE stain respectively and observe the morphology and growth situation of MSCs seeded onto the filmsResults:1.The results analyzed of these test above showed that the ester bond, carboxyl and hydroxyl groups in the PGS endowed with certain hydrophilicity and biodegradation of PGS.2.The mechanical properties, hydrophilicity and degradation of the PGS could be adjusted by altering mole ratio of propanetriol.3.The cells used in this assay had the characteristics of mesenchymal stem cells (MSCs). The average pore size, porosity and water contact angle of PGS/PLLA electrospun films was20-100μm, greater than or equal to91% and (38.4±2.5)°, respectively. The BMSCs grew with great reproductive activity and distributed on the films equally, the auxodrome of serial subcultivation MSCs was in accord with Logistic auxodrome.Conclusion:The PGS elastomer fabricated by the method of melting condensation has the mechanics in accord with soft tissues’ and good properties of hydrophility and degradation. Electronspun PGS/PLLA fibrous films with high porosity and interconnection have good performance in biocompatibility aspect as well as great potential applications in joint soft tissue engineering field.