| Objective:To observe the effects of GGA on the expression of HSF1,in order to clarify the mechanism of the expression of HSP70induced by GGA further in the rats’ retina excitotoxicity injury model induced by the injection of NMDA to the vitreous cavity.METHODS.48Wistar rats were randomly divided into4groups, including normal control group, the experimental control group A, the experimental control group B and the experimental group. No treatment was exacted to the normal control group. The experimental rats were injected GGA (800mg/kg) to the abdomen.The rats in experimental control group A and B were injected with the same amount of solvent(10%DMSO). After1hour, the rats in experimental group and experimental control group B were injected with2μl NMDA (80mmol/1L)into the vitreous body of one eye randomly and the rats in the experimental control group A were injected2μl PBS in the same way. The rats in experimental control group and experimental group were killed after2hours of the retinal excitotoxicity injury model while the rats in normal control group were sacrificed at1h. We used HE staining to observe the morphological changes of retina and used RT-PCR and immunohistochemical staining to analysis the expression of HSF1.Results:HE staining showed that the retinal structure of normal control group rats was histologically well-defined,with inerratic cell shape and uniform in dyeing.In comparison to the normal group,no obvious difference was found in the the retina structure, the shape and number of RGCs in the experimental control group A.But the retinal nerve fiber layer of experimental control group B was edema and there was vacuolar degeneration in cytoplasm of RGCs,while the number of RGCs and retinal thickness was not seen obvious difference.In comparison to experimental control group B,the damage degree of the retina in experimental group significantly decreased. The RT-PCR results showed that no evident difference was found in the expression of HSF1between normal group and experimental control group A and between experimental group and experimental control groupB.However,in comparison to the normal group and experimental control group A,the expression of HSF1increased significantly in experimental control group B. The immunohistochemical staining results showed that in normal control group and experimental control group A there was only tiny amounts of tan particles deposit on the optic ganglion cell layer, inner nucleat layer and photoreceptor-inner segment.In comparition to the prior two groups,the tan particles significantly increased in experimental control group B.In experimental group,the tan particles increased significantly in the whole layer of retina.Conclusion:1. The rats’ retinal excitotocity injury model has been set up successfully.2.In the excitotoxicity injury model,GGA can activate the activity of HSF1in the whole layer of retina and activating HSF1is the effective mechanism of GGA to increase the expression of HSP70in the retina. |
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