Study Of Cold Plasma’s Radiosensitization On Human Cervical Carcinoma Cell Hela And Human Skin Fibroblast Cell GM

Objective:By contrast the different roles of cold plasma of normal cells and cancer cells, toobserve radiosensitization effect, and to explore its mechanism of action.Method:Select normal human skin fibroblast cell GM and human cervical carcinoma cell Helaas the research object, using different conditions of cold plasma to deal with them, andthen do MTT experiment to measure cells’ growth inhibition rate. Next, we try colonyformation assay to draw cell survival curves, using flow Miriam techniques to detect cellcycle phase distribution.Results:1, With the treatment time added(about at the40s), cold plasma for GM and Helacells both show significant growth inhibition.2, Mix oxygen or increase helium flow rate could enhance the growth inhibition ofHela cells. When the working gas is argon3000ml/min: oxygen20ml/min, we achieve thebest condition. But the variation of conditions do little to GM.3,With the best condition (working gas is argon3000ml/min: oxygen20ml/min), thecold plasma do to Hela cells reach significantly stronger growth inhibition than the GMcells. Hela’s IC50is121s, while GM is194s,and the difference was statistically significant.4, Cold plasma do not produce GM cell’s radiosensitizing effect, its SER is1.03.While Hela cell is opposite, its SER is1.29. 5, Cold plasma can arrest Hela cell’s cycle in G2/M phase, join with ray can beachieve stronger effect, while GM cell has no such characteristics.Conclusion:1, Cold plasma do growth inhibition to both Hela and GM cells, influenced by theprocessing time and working gas composition.2, Cold plasma do radiosensitizing effect to Hela cells, while non-radiosensitizationto GM cells.3, Cold plasma can regulate the cell-cycle distribution, increasing the proportion of G2/M phase of Hela cells, and showed an additive effect with radiation. This may be one ofthe radiosensitization mechanism.4, Cold plasma do nothing to the cell-cycle distribution of GM cells.5, Cold plasma is expected to become the ideal radiation sensitizer.