Effects Of Valproic Acid On γ-globin Gene Expression In Erythroid Cells Cultured In Vitro

Objective To investigate the effects of virtual processor area (VPA) inperipheral blood erythroid cells of normal human onγ-globin gene expressionand its cell proliferation, and provide a new way for β-thalassemia genetherapy.Methods erythroid cells were treated with different concentrations ofvalproic acid（0mmol/L、0.5mmol/L、1.0mmol/L、2.0mmol/L、3.0mmol/L）for24h,48h,72h, respectively. Morphological characteristics of erythroid cellswere observed with light microscope, survival rate of erythroid cells weredetected by CCK-8reagents, erythroid cell colony was cultured withmethylcellulose medium, and the effects on colony forming ability of erythoidcells were viewed in different concentrantions of valproic acid. Real-timequantitative reverse transcription polymerase chain reaction (RT-PCR) wasapplied to the detection of γ-globin gene expressive changes. Results valproicacid played an inhibited role on erythroid cells and their colony forming abilityand improvedγ-globin gene expression. Conclusion valproic acid can improve γ-globin gene expression of erythroid cells. erythroid cells were treated with1.0mmol/L、2.0mmol/L、3.0mmol/L concentrations of valproic acid for24hhas the better control effect，but the concentration of2.0mmol/L、3.0mmol/Lhas the obvious inhibitory effect. So, choose the VPA1mmol/L concentrationsof valproic acid for48h/L can be obtained the best gene regulation effect.