Study On The Distribution Of The Type Of HPV Infection Among The Normal Women, The Patients Of Cervical Intraepithelial Neoplasia And The Patients With Cervical Cancer In Jinan City

Objectives:To access the distribution of the type of HPV infection among the normal women, the patients of cervical intraepithelial neoplasia and the patients with cervical cancer in Jinan city, and explore the distribution difference of high-risk HPV infection in different age group with high risk factors in jinan city. The correlation between common genotypes of HPV infection and the degree of cervical lesion was also analyzed in this study.Method:A total of9976individuals were recruited who taking HPV detection in.^department of gynaecology of jinan central hospital from March2009to March2013, among which1618ones were positive. We performed liquid-based ThinPrep cytology test(TCT) in combination with the HPV genotyping detection for the differentiation of HPV-positive patients. Colposcope and histopathologic biopsy were used to the HPV-positive women with atypical squamous cells or worse. Retrospective analysis were used to demonstrate the correlation of HPV genotyping and disease for the patients of cervical intraepithelial neoplasia and other higher-grade cervical lesions.Results:1. HPV positive rate was16.22%in jinan, in which high-risk HPV (HR-HPV) positive rate accounted for11.73%, and low-risk HPV (LR-HPV) positive rate was7.96%.The five top HPV types were as follows (in descending order):HPV16,18and33, while LR-HPV ones were HPV11,6. 2. HPV infection mostly occoures in women aged25to34(17.6%), with the age growing the HPV infection rate decreases. The HR-HPV infection occurres between the two age groups, age25to34(13.8%) and45to54(13.2%). LR-HPV infection appears its peak age before25years old (9.4%), and then gradually declines with the growth of the age.3. HR-HPV positive rate increases with the degree of cervical lesions rises, while the level of cervical lesion has no significant correlation with age.4. The types of HPV among the different pathological grade were as follow (in descending order):HPV16,18,33(in cervical cancer), HPV16、18、58、33、56(in CIN Ⅲ),HPV16、18、31、58、52、56、66(in CIN Ⅱ), and HPV16、18、59(in CIN Ⅰ).5. The number of patients with single HPV infection is more than those with multiple infection in every age group among which the peak age is25-30in both single and mutiple infection group.With the age growing the infection rate declined, and people over the age of55years appears the lowest incidence of HPV infection.Conclusions:1. The most familiar HR-HPV subtypes and LR-HPV subtypes in Jinan are HPV16, HPV18, HPV33and HPV11, HPV6respectively. In the present study, we first found out the distribution of high-risk HPV subtype in different grades of cervical lesions, which were as follows according to the detection rate from high to low: HPV16, HPV18and HPV33in cervical cancer; HPV16, HPV18,HPV58,HPV33and HPV56in CIN Ⅲ; HPV16, HPV18, HPV31, HPV58, HPV56and HPV66in CIN Ⅱ; HPV16, HPV18, HPV59in CIN Ⅰ. It is the first generation of HPV prophylactic vaccine that applies to women in Jinan area to inoculate in order to prevent cervical cancer.2. HPV infection was associated with age. It had reached a peak at the age of25～34, and after then decreased with age. HR-HPV infection rate peaked at two age groups:25～34and45～54. Unlike HR-HPV, LR-HPV infection peaked before25and then decreased with age.3. HR-HPV infection rate increased with the the degree of cervical lesions higher. Cervical cancer and precancerous lesion is closely related to the HR-HPV infection. 4. Single infection is the main form of HPV infection. Multiple HPV infection has no direct association relation with the incidence of cervical cancer and precancerous lesions.