The TCM Syndrome Distribution Regularity Of Bronchial Asthma And The Experimental Research Of Asthma Flat Granules On Bronchial Asthma Rat Lung Tissue Bcl-2、Bax Levels

1．Clinic study1.1Object: In order to explore TCM syndrome distribution regularity of asthma andit’s clinical control, provide better theory basis for the clinical treatment of asthma.1.2Method: Consulting relevant literature, combining with the experience of clinicalconfirmed mentor, according to TCM syndrome study of asthma and epidemiologicalinvestigation requirement, make asthma clinical questionnaire, this study surveys peoplewith asthma139cases, and summarize the triggers of129cases of asthma patients(inhalation, climate change, diet, tiredness, etc.), gender, age, physical condition, to livewith asthma type, the relationship between the disease and statistical analysis of relatedfactors, to research the TCM syndrome of asthma study of distribution.1.3Results: To meet the criteria for the129cases of asthma,53cases are thecold-type, accounted for41.1%, the most rare. In all cases of asthma,the male accountfor69cases,accounted for53.5%; female account for60,accounted for46.5%,incidence of women is slightly lower than men.It conform that patients with asthmasyndrome type,the physical and incentive factors are relevant.2. Experimental study2.1Object: To study Asthma ping granule on asthma model rats lung tissue apoptosisrelated genes the Bcl-2, Bax influencing the expression level, and to explore the asthmaflat infusion of mechanism for the treatment of asthma.2.2Methods:60healthy male rats, were randomly divided into6groups.10ratsevery group. The control group, Asthma model group, Guilongkechuanning group,theXiaochuanping powder high-dose group, middle-dose group and low-dose group. Afteradaptive feed one week, we began to experiment. Blank group rats with excluding OVAsaline instead of ultrasonic atomizer inhaled with2%OVA fluid. the rest of the group inthe experiment on1,8and15days, sensitization by ten sensitization method. After1week later that sensitization3times, put the rats into homemade not completely closedglass atomization box (60cm x40cm x60cm).Put10rats each time, daily inhaled ovalbumin2%saline about20min, induced asthma attacks. Inspire1times a day,stimulate14consecutive days. Asthma rat model after the success of the excitation,since the beginning of the21days, continuous14days, before the excitation of30min,each group according to the dose lavage,ping infusion of asthma group、Guilongkechuanning group rats before the excitation of30min,every groups withasthma ping granule (low dose group was0.157g/kg/d,equivalent to amount of adultdosage3.13times, middle dosage group was0.313g/kg/d,equivalent to amount of adultdosage6.25times、high dose group was0.625g/kg/d, equivalent to amount of adultdosage12.5times) and Guilongkechuanning group (0.313g/kg/d) to fill the stomach, atotal of2weeks.In order to explore the influence of asthma ping granule in treating asthma, ourteam observe the asthma flat infusion of bronchial asthma ping infusion of lung tissue ofasthma rats model of asthma rats lung tissue apoptosis related genes the Bcl-2, Baxlevels.2.3Results:2.3.1Biological characteristics observed in each group of ratsModel group rats eating less, depressed, growth retardation, dry color, less activity,oral cyanosis, dark purple tongue; Blank group rats normal diet, sensitive and active,strong hypertrophy, fur good gloss, red tongue; Drug intervention group overallsituation worse than blank group rats, loss of appetite, slow, the symptom such as darkred tongue, as a whole has changed for the better after the treatment. Rats in thebuilding after successive cough, sneeze, be agitated, difficulty breathing, noddingbreathing, nasal moist, respiratory secretions increase, audible and airway wheezingsound obvious, even convulsions. Model group rats symptoms persist; The treatmentgroup rats symptoms have improved markedly in the model group.2.3.2Granules on the horizontal level of asthma in asthmatic rat lung tissue changes inBcl-2.The Bcl-2levels: Normal group of rat lung tissue Bcl-2positive area ratio is low, the model group rats lung tissue Bcl-2positive area is significantly higher than normalgroup (P <0.01); Compared with model group, the treatment group rats lung tissue Bcl-2positive area is reduced, the P value was statistically significant (P<0.05), asthma,high dose group of rat lung tissue Bcl-2expression significantly below GuiLong tocough and gasp the ning group (P <0.01).2.3.3Changes of Bax in asthmatic rats level ofAsthma ping granuleBax levels: Normal group of rat lung tissue Bax positive area is higher, the modelgroup rats lung tissue Bax positive area is significantly lower than normal group (P<0.01); Compared with model group, the treatment group rats lung tissue Bax positivearea increases, the P value was statistically significant (P<0.05), asthma, high dosegroup of rat lung tissue Bax positive area is significantly higher than GuiLong to coughand gasp the ning group (P <0.01).3.Conclusion:3.1From129cases of asthma syndromes survey, we found that the type of cold asthmais the most common type, and shortness of breath, pockets of breath chung, full ofboring, such as plug, no sweat, XiongGe annoyed and not thirsty have greater incidenceof symptoms.3.2Asthma flat granules can inhibit airway hyperresponsiveness in asthmatic rats,improve respiratory function, and can reduce the expression of antiapoptotic proteinBcl-2, and increased expression of pro-apoptotic protein Bax, dynamic coordinationbetween the two balance, and thus relieve bronchospasm in asthmatic rats, the dialecticof clinical asthma medication has far-reaching significance.