| Background and Objective：Lung cancer is a leading cause of morbidity and cancerdeath in the world and its five-year survival rate less than15%.NSCLC account for nearly85%of all the lung cancer cases. The high mortality rate is mainly the lack of earlydiagnosis marker.DNA methylation is closely related to the development of lung cancer,therefore,it is important to research the relationship between DNA methylation andNSCLC.Dab2plays an important role in the pathogenesis of multiple tumors,however,Theexpression and DNA methylation of Dab2in NSCLC was clear.The purpose of this studyis main to study the relationship between Dab2,the DNA methylation status of its promoterand the expression of its mRNAand protein,and NSCLC.Methods：In this study, we based on four cell lines (HBE, A549,95C,95D) as well as20pairs of NSCLC tissues and its corresponding normal tissues, used real-timequantitative PCR (Real-Time PCR) method to detect the expression of Dab2’s mRNAusing β-ctin as control, detecting the expression of Dab2’s protein, using the method ofsodium bisulfite sequencing(BSP) detected DNA methylation state of Dab2in20pairs ofNSCLC tissues and its normal tissues.Results：In the four cell line the expression of mRNAand protein of HBE were higherthan A549,95C,95D.In the20pairs of sample, mRNA and protein expression in19pairsof normal tissue were higher than the corresponding NSCLC tissues, one case was inversed.The-86bp to226bp promoter region of Dab2contained23CpG sites.The methylation of17pairs of NSCLC tissue were higher than the corresponding normal tissues, two caseswere equality, one case was opposite.Conclusion：The results showed that the expression of Dab2of mRNAand protein innormal cell lines and tissues was higher than three NSCLC cell lines and the correspondingtumor tissues, down-regulation of Dab2was was closely related to the development and progression of NSCLC,the-86bp to226bp promoter region of Dab2’s methylation wasclosely related to the development and progression of NSCLC. |
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