Microbial Diversity Research Of Saliva From Caries Yugurs And Han

Objective:To analysis cariogenic bacteria microflora differences between Han and Yugurs, and the characteristics in Han, and find the possible cariogenic bacteria in this area, by research the saliva microbial community structure of in caries-active (CA) and caries-free (CF) group of Han and Yugurs in Gansu province. According to the diversity characteristics and causes of the possible pathogens about this regional group, further analyze the possible causes of dental caries, and take appropriate preventive and therapeutic measures against possible pathogens, therefore to reduce the incidence and prevalence of dental caries, consequently to improve the living quality of local human.Methods:Population in Yugurs areas of Gansu were for this study, based on16S r DNA clone library sequencing, taking nine saliva samples of local resident population and extracting the total DNA, PCR amplification of16S rDNA fragment, the fragment was transformed into E. coli DH5a, then amplified and sequenced. Comparing in the NCBI database, after dealing the obtained sequence with removing vector and heterozygous. MOTHUR software was used as a tool to analysis of microbial flora diversity, MEGA software as phylogenetic tree analysis, PCA analysis, Mann-Whitney test and other statistical analysis.Result:(1)Nine saliva samples of caries-free (CF) and caries-active (CA) group were from Han and Yugurs, there were about1014clones obtained, and got912after sequencing and screening, each library contained about300sequences, the length was about800bp. We detected109OTUs, belonging to6phylums,9classes,10orders,13families,20genera from all the samples.(2) Five differences genera with caries-active (CA) which Yugurs group with more relevance ratio than the Han group (P<0.05), moreover were both dominant genera (relevance ratio>1%), including Granulicatella, Morococcus, Streptococcus, Abiotrophia, Gemella; Five differences genera with caries-active (CA)which the Han group with more relevance ratio than the Yugurs group (P<0.05), moreover were both dominant genera (relevance ratio1%), including Veillonella, Leptotrichia, Porphyromonas, Mannheimia, Rothia, Haemophilus.(3) The principal component analysis (PCA) about caries-active(CA) of Yugurs and Han group, sample analysis shows that the two nations saliva microorganisms were concentrated in two different areas, indicating that there may be differences between their microbial composition.(4) Five differences genera in caries-active (CA) group which with more relevance ratio than caries-free(CF)group in Han (P<0.05), moreover were both dominant genera (relevance ratio1%) and including Streptococcus, Prevotella, Granulicatella, Rothia, Gemella; Six differences genera which caries-free(CF) group with more relevance ratio than caries-active (CA) group in Han (P<0.05), moreover were both dominant genera (relevance ratio>1%), including Neisseria, Porphyromonas, Veillonella, Leptotrichia, Haemophilus, Mannheimia.(5)13genera were detected in the three Han caries-active(CA) and three caries-active(CA) Yugurs samples, and the relevance ratio was both more than1%,, including Granulicatella, Morococcus, Streptococcus, Abiotrophia, Gemella, Fusobacterium, Anaeroglobus, Veillonella, Leptotrichia, Porphyromonas, Mannheimia, Rothia, Haemophilus.(6)13genera were detected in the three Han caries-free(CF) and three caries-active(CA) samples, and the relevance ratio were both more than1%, including Streptococcus, Prevotella, Granulicatella, Rothia, Gemella, Morococcus, Aggregatibacter, Neisseria, Porphyromonas, Veillonella, Leptotrichia, Haemophilus, Mannheimia.Conclusion:We analyzed the relationship between saliva microorganisms with the development of caries solid areas Yugurs and Han from the macro microflora perspective, recognizing that there may be some differences between Yugurs and local Han about the cariogenic bacteria, and Han caries pathogens maybe also differ from previous studies. Lactobacillus, Streptococcus mutans, Streptococcus sobrinus and other recognized cariogenic bacteria may not be the mainly cariogenic bacteria of local high caries population, indicating that there may not exist necessarily linked. Phylogenetic tree analysis can be used as an important means of oral microbial diversity and evolutionary relationships.