Bromocriptine Effects On MMQ Pituitary Adenoma Cells PTTG And VEGF Expression Levels

Objective To explore bromocriptine inhibited PRL secrete in MMQ pituitary tumor cells, and the effect of transforming gene (PTTG) and vascular endothelial growth factor (VEGF) expression. Preliminary discussion on the mechanism of action of bromocriptine whether dependent on change in the expression of the PTTG and VEGF factors.Methods MMQ cell culture in vitro, using a modified MTT method bromocriptine half inhibitory concentration IC50; MMQ cells groups with different concentrations of bromocriptine, the cell supernatants PRL concentrations detectded by ELISA;RT-PCR to detect the expression MMQ cells of PTTG and VEGF mRNA.Results The IC50of bromocriptine48h on MMQ cell was0.42μg/ml; With the increase of drug concentration and drug prolonged, The concentration of PRL on MMQ cell showed a trend of gradual decline;RT-PCR analysis can be seen in the different concentrations of bromocriptine intervention after48h, The expression levels of PTTG and VEGF mRNA decreases in MMQ cells.Compared with the control group, P<0.05, the difference was statistically significant. Conclusion Bromocriptine can effectively reduce the PRL level, at the same time make the mRNA of PTTG and VEGF expression decreased. We consider that PTTG is a key factor of bromocriptine reduced tumor volume, PTTG in bFGF (basic fibroblast growth factor) synergy formed by PTTG-VEGF pathway inhibition of tumor angiogenesis regulation, so as to reduce the tumor volume effect.