Promoter Region460T/C Single Nucleotide Polymorphisms In VEGF Gene And Nasopharyngeal Carcinoma Susceptibility In Guangxi Population

Background and Purpose: Vascular endothelial growth factor， also known asvascular permeability factor， is now known as the angiogenesis factor of thestrongest activity and the highest specificity. Its specific role in vascularendothelial cells is to accelerate endothelial cell migration, proliferation and isrelated to the formation of new blood vessels and lymphatic vessels. It isinvolved in new tumor blood vessels, promoting tumor proliferation andmetastasis, and the regulation of the immune system, helping tumor cells escapethe surveillance of the immune system. The present study showed that thenasopharyngeal carcinoma associated with VEGF gene polymorphisms ofmultiple sites. The crowd in guangxi as the research object, the article exploredthe relationship between promoter region460T/C single nucleotidepolymorphisms (SNP) in vascular endothelial growth factor (VEGF) gene andnasopharyngeal carcinoma(NPC) susceptibility in Guangxi population. Methods: The research object of this case-control study was divided intotwo groups,240in NPC group and245in healthy control group. We kept therecords of the two groups data. There was no genetic connection between twogroups. We drew peripheral wenous blood of the research object on an emptystomach early in the morning, the NPC group before the treatment. The bloodwas anticoagulated by sodium citrate, then preserved under the condition ofminus20degrees Celsius. We amplificated the objective gene segment usingpolymerase chain reaction (PCR) sequence specific primers method, detectedVEGF-460-T/C single nucleotide polymorphism in two groups by directsequencing method, and serum EB virus capsid antibody IgA by enzyme linkedimmunosorbent assay (ELISA) after DNA was extracted in peripheral bloodleukocytes of two groups. The genotypic distributions in the healthy controlswere analyzed by Hardy-Weinberg equilibrium. The healthy control group was arepresentative group if P＞0.05. All datas used SPSS19.0software for analysis.It was statistically significant with P＜0.05. Chi-square test was used to analyzethe general information of the two groups statistically. We calculated odds ratioand95%confidence interval of relative risk degree with correction factors suchas gender, age, nation, smoking status, EB-VCA-IgA antibody and familyhistory of NPC, using multi-factor unconditioned Logistic regression method.Results:1. The distributions of VEGF-460genotype T/T、T/C、C/C in thehealthy control group were63.3%，32.2%，4.5%. They were in line with theHardy-Weinberg equilibrium（P＞0.05）.2. The numbers of people with NPC family history were33and19in theNPC group and the healthy control group, while the frequencies ofEBV-VCA-IgA were87.1%and15.1%. There were statistical significances in distribution differences of NPC family history and EBV-VCA-IgA in twogroups(P＜0.05）. That NPC family history and EBV-VCA-IgA are risk factorsfor nasopharyngeal carcinoma.3. The distribution of VEGF-460T/T(52.9%) and T/C+C/C(47.1%)differed from those(63.3%,36.7%) in control group(χ2=5.335， P=0.021).Compared with control group(20.6%), the frequency of C allele in NPCgroup(27.5%) was significantly higher(χ2=6.303，P=0.012). Compared with Tallele, the C allele might increase the risk of nasopharyngeal carcinoma(OR=1.458，95%CI：1.115-1.952). Furthermore, compared with genotype T/T,the genotype T/C+C/C containing C allele increased the risk of lymphaticmetastasis （OR=2.214，P=0.038）.Conclusion: EB-VCA-IgA antibody is a risk factor of NPC, as well asfamily history of NPC. There is significantly correlation between polymorphismin VEGF-460T/C and NPC susceptibility. the risk of lymphatic metastasis。However， the specific molecular mechanism still needs more researches.