Polygala On Manganese Poisoned Mice The Influence Of Learning Memory Ability And Neurogenesis

Objective: To explore learning and memory in mice polygala onmanganese poisoning and the influence of neurogenesis, preliminarily polygalaintervention effect on the prevention and treatment of manganism, providingtheoretical basis for the prevention and control of manganese poisoning andexperimental basis.Methods: choose clean level60only adult female kunming mice, weight20±2g, normal control group(CG), manganese poisoned group(MG),manganese poisoned with polygala high dose group (polygala high-dose group,MHG), manganese poisoned with polygala middle dose group(polygalamiddle-dose group, MMG), manganese poisoned with polygala low dose group(polygala low-dose group, MLG)， a total of5groups,5groups of12.Intraperitoneal injection of manganese chloride (MnCl215mg/kg) in a way thatMG, MHG, MMG, MLG four manganese poisoning mice and normal controlgroup (CG) by intraperitoneal injection of the same dose of saline (NaCl),thereby establishing manganese poisoning mouse model last three weeks by intraperitoneal injection of manganese chloride. At the same time, giving MHG,different concentrations of MMG, MLG three groups of mice fed Polygala,Polygala high-dose group (MHG) at a concentration of1g/mL, by10g/kgorally; Polygala dose group (MMG) at a concentration of0.5g/ml, accordingto5g/kg orally; polygala low dose group (MLG) to a concentration of0.25g/mL,2.5g/kg orally, Polygala gavage for4weeks. In the four weeks raised fivemice in the same environment. After4weeks in Morris water maze test itsspatial learning and memory ability, detected in the hippocampus CA1, PKAexpression CA3region, AKT expression CA1region, immunofluorescencebrain SGZ and SVZ DCX expression by immunohistochemical methods.Calculate the number of cells positive image, and using Image-ProPlus6.0image analysis system in the hippocampus CA1, PKA mean optical density CA3region, brain SGZ and average optical density values, SVZ DCX-positive cells.Morris water maze for all data, optical density, and number of positive cellswere obtained using SPSS16.0software for statistical analysis and correlationanalysis.Results:1.in Morris water maze test in, MG and CG comparison, theescape latency was prolonged, the difference was statistically significant(P<0.05), MHG, MMG, MLG compared with MG, the average escape latencydecreased(P<0.05), compared with the CG, little change in the average escapelatency, the difference was not statistically significant (P>0.05).2in spaceexploration experiments, MG compared with the CG, reduce the number ofcross platform (P<0.05), MHG, MMG, MLG compared with MG, the numberincreased significantly across platforms(P<0.05), compared with the CG, littlechange in the number of cross platform(P>0.05).3hippocampal CA1and CA3regions chemistry results PKA immunohistochemistry Show: MG compared with the CG, reduced expression of PKA, there was a significant difference (P<0.05); compared MHG, MMG, MLG and MG, increased expression of PKA(P<0.05); MLG comparison with CG, reduced expression of PKA(P<0.05); nosignificant difference (P>0.05) between MHG, MMG and CG. DCXimmunofluorescence4.SGZ and SVZ display: MG compared with the CG,DCX expression was decreased(P<0.05); compared MHG, MMG, MLG and MG,DCX expression increased(P<0.05); MLG compared with the CG, DCXexpression was decreased(P<0.05), MHG, MMG compared with the CG(P>0.05).5hippocampal CA1chemistry results AKT immunohistochemistryShow: MG compared with the CG, AKT expression was decreased(P<0.05);MHG, MMG, MLG compared with MG, AKT expression increasedsignificantly sex (P<0.05), compared with the CG(P>0.05).6linear correlationanalysis showed that, PKA, DCX-positive cells with mouse memory capacity(the number of cross platform) were positively correlated, PKA-positive cellswithin the SGZ DCX-positive cells were positively correlated.Conclusion:1.Polygala manganese poisoning mice intervention canimprove learning and memory.2.Polygala intervention can promote manganesepoisoning mouse brain neurogenesis3.Manganese poisoning reduces theexpression of PKA, AKT mouse brain.4.Polygala intervention can enhance theexpression of hippocampal PKA, AKT manganese poisoning mice. Mechanism5. Polygala manganese poisoning intervention to improve learning and memoryin mice may upregulate the expression of PKA Polygala and promote neurogenesis related.