Clinical Influences Of Class Ⅲβ-tubulin, Sox2and Survivin Expressions On Locally Advanced Ovarian Cancer Patients Receiving Taxane-based Or Platinum-based Chemotherpy

ObjectiveOvarian cancer is usually associated with chemoresistance and poor survival. Although Class Ⅲ β-tubulin, Sox2and Survivin have been linked to poor prognoses in many cancers, few definite conclusions could be firmly drawn among ovarian cancer.MethodsA total of110stage Ⅲ ovarian epithelial cancer patients were divided into Taxane/Platinum group (58cases) and Platinum-based group (non-Taxane based,52cases) following primary cytoreductive surgery. Protein expressions were evaluated by immunohistochemistry. Results were analyzed in relation to clinicopathological characteristics, response to chemotherapy, progression-free survival (PFS) and overall survival (OS). Besides, expressions of Class Ⅲ β-tubulin, nuclear Survivin and cytoplasmic Survivin were analyzed according to Sox2status.Results1. Positive expressions of Class Ⅲ β-tubulin and Sox2in110cases were59.09%and61.82%, respectively. Positive expressions of Survivin wrer observed with60.34%cases only in nucleus,15.52%cases only in cytoplasm and24.14%cases in both nucleus and cytoplasm.2. Expressions of nuclear Survivin and Class Ⅲ β-tubulin were significantly in line with that of Sox2(p=0.005and0.020, respectively).3. In Taxane/Platinum group, positive expressions of Class Ⅲ β-tubulin, Sox2and only nuclear Survivin were significantly associated with resistance to chemotherapy (p=0.006,0.007and0.009, respectively).4. In Taxane/Platinum group, Kaplan-Meier method showed cases with positive expressions of Class Ⅲ β-tubulin, Sox2and only nuclear Survivin obtained a shorter PFS than negative expression (p=0.032,0.005and0.004, respectively). The Univariate model indicated residual tumor>2cm, Class Ⅲ β-tubulin, Sox2and nuclear Survivin positive expression showed a shorter PFS (p=0.001,0.042,0.009and0.047, respectively). The Multivariate model indicated residual tumor>2cm, Class Ⅲ β-tubulin and Sox2retained the unfavorable independent prognostic variables for PFS (p=0.002,0.038and0.047, respectively).5. In Platinum-based group, the expressions of Class Ⅲ β-tubulin, Sox2and Survivin were not related to the response to chemotherapy and PFS (p>0.05).6. The expressions of Class Ⅲ β-tubulin, Sox2and Survivin were not correlated with OS (p>0.05).7. The significant independently factors for predicting OS were response to chemotherapy, histotype and residual tumor size (p<0.0001, p=0.002and0.010, respectively).Conclusions1. Positive expressions of Class Ⅲ β-tubulin, Sox2and nuclear Survivin were unfavorable independent prognostic factors for Taxane resistance in locally advanced ovarian epithelial cancer.2. Evaluating the combined expression of Class Ⅲ β-tubulin, Sox2and nuclear Survivin may improve the predictive accuracy for chemotherapy resistance.3. Positive expressions of Class Ⅲ β-tubulin, Sox2and nuclear Survivin were predictive for poor PFS in locally advanced ovarian epithelial cancer patients receiving Taxane-based chemotherapy.4. The expressions of Class Ⅲ (3-tubulin, Sox2, nuclear Survivin and cytoplasmic Survivin were not correlated with OS in locally advanced ovarian epithelial cancer patients.5. Prognostic factors for OS were response to chemotherapy, histotype and residual tumor size in locally advanced ovarian epithelial cancer patients.