Different Concentrations Of Etomidate Induction Of Human Hepatocellular Carcinoma HepG2Cells Apoptosis In Vitror

ObjectiveEtomidate, as one of the most common anesthetic drug, has been widely used inhospital. With the continuous development of anesthetic techniques, the rates ofsurvival patients with malignant tumor also increased. People begin to pay attentionto whether anesthetic techniques and the anesthetic drug have an effect on the tumorimmune system. Clinical found that long-term use of an etomidate it can causeimmune suppression and increase the cytotoxic effect. Etomidate can inhibit theHL-60cell activity directly, and induce the apoptosis of the cells, then whetheretomidate directly induced HepG2apoptosis in cancer of the liver. We based thisstudy hypothesized,Therefore,this article designed to investigate the differentconcentrations of etomidat whether directly induce the cell apoptosis to seek open upnew ways of etomidate clinical treatment.MethodsHuman liver HepG2cell line were amplificated cultivation for use invitro,choose different concentrations of etomidateE1(0.5ug/ml),E2(5ug/ml)andE3(50ug/ml)(blank) as control group, HepG2were observed respectively after12h,24h,and48h.With liver cancer HepG2cell lines as target cells,· Annexin V/FITCfluorescence probe, flow cytometry instrument to analysis cells apoptosis, its valueuse mean+/-standard deviation, the results of each group according to the tworesearchers repeated measurement twice to proofread.ResultsCompared with the blank control group,12h,24h,48h after culture E1and E2group liver HepG2cell line apoptosis rate has no obvious difference (P>0.05), E3group of apoptosis rate increased, and with significant difference (P <0.05); As the extension of incubation time and concentration increasing, E1and E2groupcompared with liver HepG2cell line apoptosis rate have no significant difference (P>0.05), E1and E3group compared with liver HepG2cell line apoptosis rate havesignificant difference (P <0.05), E2and E3group compared with liver HepG2cellline apoptosis rate have obvious difference (P <0.05). This prove that etomidateinduced liver cancer HepG2cell line apoptosis has a certain concentration-and time-effect relationship.ConclusionEtomidate with safe and effective concentrations in clinical are not directlyinduced HepG2liver cancer cell apoptosis in vitro, but when concentration and timereach a certain peak can be directly induced the hepatocellular carcinoma HepG2cells apoptosis in vitro.