The Mechanism Of Total Alkaloid Of Sophora Alopecuroides On Treating Experimental Colitis Of Rats

Obeject:To study the mechanism of action about treatment of total alkaloid of Sophora alopecuroides(TASA)on rats with experimental colitis.Methods:1.To observe the treated effects of TASA on pathological change of rats with experimental colitis In the 7th day and the 21th day,the experimental colitis model of rats was induced by administrating 2,4,6-trinitrobenzesulphonic acid (TNBS)/ethanol rectally,the symptoms and colonic histology were evaluated.2.To observe the effects of TASA on the expression of CD4~C+D25~+Tr、CD8~+CD28~- Tr of rats with experimental colitis by FCM.3.To observe the effects of TASA on IL-10 cytokine of rats with experimental colitis by ELISA and RT-PCR.4.To respectively study the correlation between the expression of CD8~+CD28~-Tr and DAI,between the expression of CD8~+CD28~-Tr and histological grading of colitis in the colon of rats and between the expression of CD4~+CD25~+Tr and IL-10 cytokine in the colon and peripheral blood(PB)of rats.Results:1.A significant decrease was found in DAI and Histological grading of colitis of normal group in the 7th day and the 21th day and all TASA groups, compared with model group(P＜0.01),could alleviate the symptoms and histological damages of model rats in the 21th day(P＜0.05).2.①In the 7th day after modelling: The proportion of CD8~+CD28~-Tr and CD8~+CD28~-/CD28~+ in the colon of colitis rats of model group was higher than that in normal control group,but the proportion of CD4~+CD25~+Tr,CD4~+CD25~+/CD25~- is lower than that in normal control group (P＜0.01).Compared all TASA groups with model group,the statistical differences were not significant(P＞0.05).②In the 21th day after modelling:The expressions of CD8~+CD28~-Tr and CD8~+CD28~-/CD28~+ Tr in the PB and colon of rats of model group were obviously higher than those in normal control group and all TASA groups(P＜0.05),and the expressions of CD4~+CD25~+Tr and CD4~+CD25~+/CD25~- in the colon tissues were lower than those in normal control group and all TASA groups(P＜0.01).③Growth rates of colon’s CD8~+CD28~-Tr expression of model,5-ASA and all TASA groups obviously are higher in the colon of colitis rats than that of PB(P＜0.05).3①The expression oflL-10 cytokine in the colon and the PB of colitis rats was lower than that of normal control group(P＜0.01),moreover, the expression in the colon tissue was the lowest of them.The expression of IL-10 cytokine in the rats with TASA was almost as well as that of normal control group.4 The positive correlations were demonstrated between the expression of CD8~+CD28Tr and DAI(Pearson r=0.828,P＜0.01),between the expression of CD8~+CD28~-Tr and histological grading of colitis(Pearson r=0.819,P＜0.01),also existed CD4~+CD25~+Tr and IL-10 cytokine in the colon of rats(Pearson r=0.789, P＜0.01),whereas,no correlation was found between CD4~+CD25~+Tr and IL-10 cytokine in the PB of rats(r=-0.191,P＞0.05). Conclusions:1.TASA could significantly alleviate the pathological changes of rats with experimental colitis,which can obviously reduce exfoliation or necrosis of mucosa and decreased inflammatory cell in the mucosa and mucosa underlayer on the rats with colitis.The colon only contain a few small fibroplasias or ulcers.2.TASA could upgrade the expression of CD4~+CD25~+Tr and degrade the expression of CD8~+CD28~- Tr of rats with experimental colitis,relieve the inflammatory reaction.3. TASA could significantly regulate the expression of IL-10 cytokine of UC rats, improve the symptoms.4 The positive correlations were demonstrated between the expression of CD8~+CD28~-Tr and DAI,between the expression of CD8~+CD28~-Tr and histological grading of colitis,also existed CD4~+CD25~+Tr and IL-10 cytokine in the colon fo rats,whereas,no correlation was found between CD4~+CD25~+Tr and IL-10 cytokine in the PB of rats...