A Preliminary Study G Protein β Subunit (RGB1) Function Trimer Rice Vary

Heterotrimeric G-proteins (G proteins) have been conversed as an important signal module in all eukaryotes. G proteins function as a molecular switch to regulate various of signal transduction pathways and thus to be implicated in a wide range of life progress. G proteins complex in plant is much simplier than its counterpart in mammals. Studies of plant G proteins have been more concentrated on the model plant Arabidopsis thaliana, and more on the G proteins a subunit (Ga). In this study, we identified the Gβ in Oryza Sativa (RGB1), and its function in different hormone and stress signal pathways is preliminarily discussed. Lines of transgenetic rice plants were also constructed. The major results are as follows:1. RGB1has a strong interaction with RGG1(G protein y subunit of rice), but little with RGA1(G protein a subunit of rice). The result indicates that RGGl may be an essential element for the interaction between RGA1and RGB1.2. Seedlings of Oryza Sativa was treated with different types of hormones and abiotic stresses to study the response of RGBl to them. We discovered that RGB1may be an important component of GA、NaCl、drought and cold signal pathways in Oryza sativa, especially drought.3. Cellular localization study of RGB1was performed through transfections of rice protoplasts. We observed that RGB1was located on the membrane of the cells.The results are not completely in accordance with published data, so more work needs to be done on the location of RGB1.4. Transgenic rice plants of rgb1RNAi/WT were constructed. We discovered that seeds of rgb1RNAi/WT were much smaller, compared with the wild type. And seedlings of T1generation were shorter. Hence, we indicate that RGB1plays an important role in the growing and developing stages of rice.In summary, we did some preliminary study on the function of RGB1and constructed transgenic plants as materials for the following studies. This will be helpful to understand more about the G protein pathways in rice, and to understand the differences of G protein pathways between dicotyledoneae and monocotyledon plants.