C-erbB-2 Expression And Muc1 Its Significance In Papillary Thyroid Carcinoma

Objective:To study the expression of c-erbB-2(the human epidermal growth factor receptor2) and mucin1(muc1) in papillary thyroid carcinoma and benign thyroid tissues and to explore its relationship with the clinicopathologic parameters. Study on the Feasibility of which c-erbB-2and muc1are being the thyroid malignancy molecular markers,try to look for the new method to diagnosis thyroid cancer earlily and establish a new thyroid cancer gene therapeutic targets, ang provide clues and ideas for opening up the new treatment of thyroid cancer.Methods:Immunohistochemistry streptavidin-perosidase (SP) method was used to measure the expression of c-erbB-2and mucl in125papillary thyroid carcinoma,42nodular goiter and29normal thyroid tissue.In order to use cell counting to estimate positive result, we observed staining samples by naked eye assisted by image measuring software Image-ProPlus6.0produced by the United States MediaCybernetics company. All papillary thyroid cancer samples were reclassified according to the standards of latest edition of the World Health Organization (WHO) classification of tumours of thyroid and combined with clinicopathological characteristics of malignancies of thyroid.The positive degree of c-erbB-2and mucl expression were divided into4from negative to strong positive expression relative to the recent European and American Clinical Laboratory grading standard.We Used Chi-Square Test with SPSS software package to analyze the differences of c-erbB-2and MUC1expression in papillary thyroid carcinoma between different ages and tumor sizes groups, lymph node metastasis and non-metastasis groups and different histological grades and TNM stages groups(In the event of no positive results, compared the strong positive part).The expression results of c-erbB-2and mucl were matched and the relativity was estimated by SPSS software package with Wilcoxon’s Sign Rank Test(Nonparametric2Related Samples tests).Results:1. Expression characteristics:The expression of c-erbB-2mainly located in the cell membrane of the thyroid tissue, the positive staining distributed unequally weak or strong in papillary thyroid carcinoma, the c-erbB-2was weak or no expression in thyroid benign lesions and adjacent tissues. We found mucl mainly located in the top surface of thyroid follicular, its expression increased and polar distribution disappeared, abnormal expression often appeared in the nucleus and cytoplasm. Expression of MUC1and C-erbB-2was associated with cervical lymph node involvement and clinical stage but not with age,tumor size,histologic grade or pathological typing.C-erbB-2expression was positively correlated with MUC1expression(Z=4.502,P<0.001).2. Related data:The positive rate of c-erbB-2and muc1expression in papillary thyroid carcinoma was47.20%and84.00%,respectively.It was significantly higher than the rate in nodular goiter(16.67%,61.90%) and normal tissues(6.90%,58.62%;P<0.001). The positive rate of C-erbB-2and MUC1expression of papillary thyroid carcinoma increased with TNM stags (P=0.001, P<0.05). The positive expression rate of c-erbB-2increased with thyroid cancer histological grade (P=0.003).The expression of MUC1was nonspecific to thyroid cancer histological grade, but the strong positive rate increased with thyroid cancer histological grade(P<0.05). The positive rate of c-erbB-2in non-metastasis and metastasis groups of papillary thyroid carcinoma was respectively tested as37.84%、60.78%，the metastasis group is increased remarkably compared with non-metastasis group (P=0.012), The expression of MUC1was nonspecific between the groups. There was no statistical significance between the groups of different ages and tumor sizes about the both protein antigens. In addition, the paired data showed that the positive rate of c-erbB-2was24.00%in which samples muc1appeared weak positive.47.27%in which samples mucl appeared moderate positive,68.00%in which samples muc1appeared strongly positive,the expression increased linearly with the positive degree of muc1.Wilcoxon’s Sign Rank Test(Nonparametric2Related Samples tests) was used to analysis the relativity between c-erbB-2and mucl,the outcome showed that the expression of c-erbB-2and MUC1were positively correlated (Z=-4.604, P<0.001).Conclusion:1. C-erbB-2and muc1promote the evolution of normal thyroid tissue to thyroid cancer, respectively.and both protein antigen have a synergistic effect on the evolution process of invasion and metastasis of papillary thyroid cancer cells, respectively. C-erbB-2and muc1are likely to be used as molecular markers of clinical papillary thyroid cancer.the detection of c-erbB-2and mucl will help to distinguish benign and malignant thyroid tumors and provide more information for judgment of metastatic cervical lymph nodes and thus guiding treatment and improving the prognosis.2. C-erbB-2might be therapeutic targets for treating thyroid malignant tumor.