| Aims: Effects of different intensity training on myocardial mitochondrialrespiratory chain enzyme complex activity, myocardial metabolism of free radicalsand the anti-oxidant capacity (SOD, CAT, GSH-PX activity and MDA content) in rats(wistar) were investigated in2012. From the standpoint of the change of runningsuitability of the activity of oxidase of mitochondria respiratory chain and theanti-oxidation capability and myocardial antioxidant capacity, it was investigatedpossible mechanism of adaptive changes in energy metabolism of the myocardiumunder different yocardium al arining intensity trainings in order to provide theoreticalbasis for reasonable exercise and physical activity.Methods: The experimental treatments were based on the standard of maximaloxygen of Bedford and Ding Shu zhe reported. Five treatments included(1)Control-group(CK),(2) low-intensity group(LT),(3) medium-intensity group(MT),(4) high-intensity group(HT),(5) extremely high-intensity group(HE). Fifty big malemouses (wistar) were used and divided randomly into five groups, and each group hasten mouses. The rats were fed normally for control-group treatment and training sixdays for other treatments once a day and totally eight cycles. It sustains caesareansection after batch anesthetized rats in the fasting state after the end of the eight weeksof training, each mode group quickly removed the rat samples cardiac mitochondrialrespiratory chain enzyme complexes I, Ⅱ, Ⅲ, Ⅳactivity of myocardial tissuesuperoxide dismutase (SOD), glutathione peroxidase (GSH-Px)and catalase (CAT)activity and malondialdehyde (MDA) content determination. activity in cardiac tissuesuperoxide dismutase (SOD), glutathione peroxidase catalase (CAT) activity andmalondialdehyde (MDA) content determination, the activities o f the mitochondrialrespiratory chain enzyme complexesⅠ,Ⅱ, Ⅲ, Ⅳ measured by Uv Spectrophotometry.Antioxidant enzymes SOD, GSH-PX, CAT activity and MDA content determinationusing kit were purchased from Nanjing Jiancheng Bio Co. Ltd, and operated strictlyaccording to the kit instructions.Results:(1) The body weights of rats under intensity training for eight weeks werechanged. Compared to conventional feeding (CK), reducingof body weights in extremely high intensity training group (HE) was the most, moderate intensitytraining group (MT) was less.(2) The results showed that effects of different training intensity of rat onmyocardial mitochondrial respiratory chain enzyme complexes Ⅰ~Ⅳ activityweredifferent.Compared with the quiet routine feeding CK, enzyme complexⅠactivity of MT and HE treatment were significantly higher elevated, increased168.21%and137.77%respectively, LT and HT treatments were reduced6.03%and10.07%respectively; Compared to conventional feeding, enzyme complex Ⅱ activityof HT and HE treatment were reduced66.79%and6.57%respectively,but the activityof LT and MT treatment were Respectively elevated,increased3.65%and55.47%;MT and HE mode of enzyme composite material Ⅲ activity were very significantlyincreased275.38%and218.15%, while the high intensity training mode (HT) wassignificantly with decreasing20%.Rat myocardial mitochondrial respiratory chainenzyme Ⅳactivity and enzyme activity has different changed, with highly intensitytraining mode (HE) rised biggest (223.99%), moderate intensity training mode(MT)increased the minimum (57.28%).(3) With the increase of training intensity, SOD activity increased first and thendecreased.viz: HT (14.16)> MT (11.78)> LT (11.08)> HE (10.92). Compared toCK, SOD in medium intensity training mode (MT) and high intensity training mode(HT) was higher6.1%and27.5%, respectively. And the intensity of low trainingmodel (LT) and extremely high intensity training (HE) has reduced t by0.3%and1.7%respectivelyand there was significant or highly significant difference betweenmodes (P<0.05or P<0.01).(4) With the increase of training intensity, GSH-PX activity of myocardial tissuein rat dropped first and then rose.viz, HT (104.865)> MT (104.229)> LT (82.672)>HE (72.177). GSH-PX activity in both MT and LT was higher21.05and21.69thanthat in CK, respectively. But the HT and HE showed the opposite effects, waslower0.51and11.0than that in CK, respectively. In addition, there was significant orhighly significant difference (P<0.05or P<0.01) between the different trainingintensity treatments.(5) With the intensity training increasing, the experimental results show thatCAT activity gradually increased when training intensity reached85%of maximum oxygen consumption CAT maximum, then declinedwith increasing training intensity.There are significant or very significant differences between different intensitytraining treatments and CK (P<0.05or P<0.01). The activity of CAT different trainingintensity.the differencr value between the training intensity and CK showed HT(0.294)> MT (0.223)> LT (0.178)> HE (0.093). There were significant or extremelysignificant differences between the different training intensityexcept the LT and MT(P<0.05or P<0.01).(6) There were significant and highly significant differences (P<0.05or P<0.01)between different intensity training model and CK. MDA contents in LT, MT and HTtreatments significantly increased by50.20%,11.11%and71.74%respectively, whileHE decreased3.90%. There are significant and highly significant differences amongdifferent training intensity (P<0.05or P<0.01).Conclusion:(1) After eight weeks of different strength training, four different trainingintensity mode amplitude weight gain was significantly lower than that of controlmode, embodied in: HT> HE> LT> MT, the high growth rate of body weighttraining mode, extremely high-intensity mode has affected the normal growth of ratbody weight growth, inhibit the natural growth of body weight in rats.(2) Different training intensity pattern will cause respiratory chain function in ratwere increased, but different training patterns at different levels to improve thefunction of mitochondrial respiratory chain were difference. moderate intensitytraining mode in the enhancement of rat myocardial mitochondrial respiratory chainenzyme function, improve the mitochondrial ATP synthesis capacity than the highand training mode、high intensity training mode and low intensity training mode.(3) Different intensity training on rat myocardial tissue antioxidant capacity willimpact, moderate intensity training mode and the high intensity training in improvingthe antioxidant capacity was significantly higher than that of low intensity trainingmode and high intensity training mode, extremely high-intensity mode can reduce thelipid peroxide the MDA content. |
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