Aerobic Exercise On Brain Aging And Spatial Learning And Memory In Rats And Hippocampus NCAM Expression

Objective: By D-galactose induced aging SD rats aging, theaging process60min/d of aerobic exercise intervention, the determination of SD ratbrain aging and spatial learning and memory ability and hippocampal NCAM geneexpression, to explore the aerobic exercise intervention on rat brain aging and spatiallearning and memory ability and hippocampal NCAM gene expression. Methods：64SD rats were randomly divided into four large groups: acute the Aging group (NDgroup), the saline control the group (NNa group), acute aging before the exerciseintervention group (SD group), acute aging process exercise intervention group (theNDs group). After the end of each of the large group of aging modeling, random andthen divided into two groups: a group of naturally fed for seven days, seven days ofthe Morris water maze test for N group, another group do the M group (for example:ND component for the NDN group and NDM group), a total of eight group. After drawn, determination of the rat brain SOD, GSH-PX activity and MDA content wereobserved brain aging rats; application of real-time PCR, Western blotting ofhippocampal NCAM gene expression. Results:1、General observations: the ND ratsthan NNa rats, there has been lack of energy, slow, lethargy, fur, curly brown, dullserious hair loss and other obvious symptoms of aging.2、Radicals test results: theNDN group of SOD, GSH-PX activity was significantly lower than NNaN group (P<0.01), while the the NDN group of MDA content significantly higher than NNaNgroup (P <0.01); SDN group NDN group SODGSH-PX activity and MDA contentwas no significant difference (P>0.05); NDsN group SOD activity is significantlyhigher than that the NDN group (P <0.01), GSH-PX activity was significantly higherthan the NDN group (P <0.05), MDA content is very significant resistance below thethe NDN group (P <0.01); NDsN the group and NNaN group of SOD, GSH-PXactivity and MDA content had no significant difference (P>0.05).4M groupcompared to the same groups trend with the N group. The M group compared to thecorresponding N group SOD, GSH-PX activity and MDA content were nosignificant differences (P>0.05).3、Morris water maze navigation test results: theNNaM NDM, SDM, NDsM four groups of rats the initial formation of spatiallearning and memory are after two days of training, NDM group rats four days toform a stable spacelearning memory, NNaM group formed in three days stable spacelearning and memory, SDM group memory formation instability the volatile, NDsMgroup is stable formation of learning and memory after three days of training. Thespace exploration Results: first into water quadrant NNaM rats platform across thenumber of very significant increase (P <0.01) compared with the NDM group; theSDM group compared with the NDM group, no significant difference (P>0.05);NDsM group compared to NDM group was significantly increased (P <0.05).4、Hippocampal NCAM mRNA and protein detection: NDN group compared withNNaN, hippocampal NCAM mRNA and protein expression was significantly thesexual lowered (P <0.01); the SDN group NDN groups, no significant difference (P>0.05); NDsN groupits expression level was significantly raised (P <0.01) ascompared with the NDN group; NDsN group with NNaN was no significantdifference (P>0.05).4M group group comparison of NCAM mRNA and protein in the same trend with the N group.4M group and the corresponding N group, mRNexpression was no significant difference (P>0.05) and protein expression weresignificantly raised (P <0.05).Conclusion:（1）D-galactose induced aging ratsshowed obvious symptoms of aging, the brain activity of SOD, GSH-PX activitywas significantly lower than the saline control group, MDA content was significantlyhigher than the saline control group. The proof of this experiment, D-galactoseinduced brain aging rats modeling success.(2) Aerobic exercise intervention in theaging process, can effectively delay the process of brain aging; aging movementpretreatment, the impact of brain aging no significant sexual significance, promptedaerobic exercise on the body positive impact may vary with the forward movementof the stop slowly subsided.(3) Aerobic exercise intervention in the aging processcan be maintained properly sustained expression of NCAM mRNA and protein in thehippocampus, which may be one of the mechanisms of aerobic exercise to reduce theability of learning and memory in rats recession.