Analysis Of Fluorescence Effects Of Laser - Induced Biomolecules

The laser induced fluorescence spectroscopy (LIFS) is widely used in life science. However, the explanation about micro-mechanism of LIFS is far from complete and perfect. Using quantum theory, this dissertation study the weak laser biomolecular systems of the micro-mechanism of non resonance fluorescence effect.The resonance fluorescence micro-mechanism of laser biomolecular effect is very complex, we can only get the approximate solution of the schrodinger equation. With the dressed state approximate description of biological molecules in the laser field, some laser induced effect of biological molecules fluorescence experimental results can be explained.Based on Frohlich biological boson condensation theory, the biological system was considered as a highly coherent system, with the theory of modification of the micro mechanism of laser induced effect of biological molecules fluorescence is analyzed. The relation between the fluorescence intensity has been calculated by quantum mechanics. Using the theory of laser induced fluorescence spectrum explained the experiment results, which were the absorption of whole blood spectra, NO two-photon laser induced fluorescence spectrum, plant chlorophyll fluorescence spectrum, a single DHL cell fluorescence spectrum, and laser induced blood is under different excitation wavelength excitation fluorescence spectra. The experiment result of laser induced fluorescence spectra of the blood was also explained with Forster energy transfer theory.Quantum theory analyzes the micro-mechanism of LIBFE, and come to the conclusion that fluorescence intensity is proportional to εk2, and excitation laser intensity is proportional to εk2the conclusion.