Determination Of Heavy Metal Speciation In Seafood By Ion Chromatography-Atomic Florescence Spectrometry

Along with the development of the instrumental analytical technology, the health effects of common metal elements gain more attention. In a variety of excessive incidents’emerge endlessly today, element speciation analysis technology is of great significance. Coupled with sensitive spectroscopy or mass spectrometry detection technology and preeminent chromatographic separation technology, hyphenated techniques are the inevitable trend of technological development. There are 4 chapters in this paper.Chapter 1:Cited mass of references, mainly around the speciation analysis technologies of arsenic, mercury and selenium were reviewed in this chapter, modern commonly instruments hyphenated techniques and the corresponding sample pretreatment techniques were mentioned also. The advantages and disadvantages of various methods and developing prospects were discussed thoroughly. Future prospects were made in speciation analysis and homebred instrument.Chapter 2:Ion chromatography-ultra violet-hydride generation-Atomic Florescence Spectrometry was applied to detect 5 arsenic species in seafoods. Separately adopt water, acetic acid, hydrochloric acid and formic acid to extract arsenic from bonito. The concentration of extraction reagents and instrument conditions were optimized. The results showed that samples were used 2% formic acid as extract reagent, extractive efficiency was higher than others, and the recoveries were between 72% and 110% with a relative standard deviation (RSD) of less than 5%. The 5 arsenic species were separated in 8 min. The detection limits were 3.543 μg/L for AsB,0.426 μg/L for As(Ⅲ),0.216 p.g/L for DMA,0.211μg/L for MMA and 0.709 μg/L for As(Ⅴ), with linear coefficients more than 0.9990.Chapter 3:Ion chromatography-ultra violet-hydride generation-Atomic Florescence Spectrometry was built to detect 3 mercury species in seafood. The concentration of extraction reagents and instrument conditions were optimized. In optimized conditions, Euphausia superba treated with multiple extracts were detected. The results showed that 2%(v/v) HCOOH+1%(m/v)+0.15%(m/v) KCl was the chosen extract, it has higher extractive efficiency, and the recoveries were between 95.76% and 116.10% with a relative standard deviation (RSD) of less than 5%. The method has advantages of high sensitivity, effective separation, less amount of sample and wide linear range of determination. The method has high accuracy and stability, and could be used to determine the mercury speciation in seafood.Chapter 4:The euphausia superba, as the largest single biological resources on the earth, because of its high nutritional value and potential application, known as one of research focuses today. Total selenium was detected in seafood (especially euphausia superba and its production). In this chapter, the method of selenium detection with atomic florescence spectrometry was built. The method has advantages of easy operation and time saving, with a standard linear equation of I= 35.37* C-16.75 (r2= 0.9997). AFS detection and the result was compared with ICP-MS detection. It was found that the highest selenium content in the Euphausia superba powder, selenium content in the products of euphausia superba, Euphausia superba oil is less than other samples, it is likely to be imperfect process caused the loss of selenium. Then, we carried the work of selenium speciation analysis, and found many problems such as low sensitivity and bad separation, etc.