Isolation Of Deodor Microorganism From Vermicompost And Optimization Of Culture Conditions

With the development of industry and agriculture and people’s increasing requirements for the surrounding environment, odor pollution has been paid more and more attention. The research shows that the main pollutants in the odor pollution are ammonia and hydrogen sulfide that results chronic poisoning, a variety of respiratory diseases, or even threats of losing life. As granular material with high porosity and specific surface area, vermicompost is a kind of efficient adsorbent for the odors. It contains a large number of micro-organisms which can promote the decomposition of organic matters and reach the goal of deodorizing. However, for now, domestic microbiological studies on the vermicompost is not deep enough, and research and application reported about odor treatment is also rare.This experiment uses the vermicompost which is obtained from the products of earthworm dealing with municipal sewage as the research object. Use the property of deodorization and select the efficient strains to get the strains to compound. Then test the ability of odor deodorization of the compounding strains and come to a conclusion after optimizing the conditions of compounding strains:Assisted by high-throughput sequencing methods to select the deodorant microorganism, the sequencing results shows that it has alkaligenes which capable of degrading ammonia and bacillus which is better at degrading sulphur.With the combination method of preliminary screening and secondary screening, after 25 days of fostering, separate 6 efficient ammonia degrading strains NH1、NH4、NH6、NH9、NH11、NH12 and NH13 and 5 desulphurizing bacteria strains HS2, HS3, HS5, HS7 and HS10 from the vermicompost. Among them, the most efficient ammonia degrading strains are NH1 and NH3 and the rate is 50.34%. The most efficient desulphurizing bacteria strains are HS3,H2 S and the rate is 54.69%.Compound the ammonia bacteria and desulfurizing bacteria respectively after the secondary screening and use the compounding strains in the biodegradation experiment of chicken manure. And the the results show that after 25 days, combination of NH1 and NH9、NH11 makes the amount of NH3 releasing reduce by 58.21% compared with the control group. The combination of HS2, HS3 and HS5 makes the amount of H2 S releasing reduce by 72.55% compared with the control group. Under the same condition, combination of three strains is more efficient than a single one.Have a physiological and biochemical and molecular biology identification to the 6 compounding strains respectively. The result shows that: NH1 is Alcaligenes sp; NH9 is Citrobacter sp; NH11 is Pseudomonas sp; HS2 is Arthrobacter sp; HS3 is Stenotrophomonas sp; HS5 is Bacillus sp.The result of mixed fostering optimization on the compounding strains reveals: the best condition for fostering compounding strains of degrading ammonia is 30 ℃, 7.5 p H, 175 rpm of rotation speed and 10% inoculum size. The best condition for fostering compounding strains of desulfurization is 28 ℃, 7.0 p H, 150 rpm of rotation speed and 15% inoculum size.