Synthesis, Structure, Antibacterial Activity And DNA Lysis Activity Of 4- (1H-1,2,4-triazole-1-methylene) Benzoic Acid Transition Metal Complexes

Triazole-based compounds display striking biological antifungal properties and good chemical characteristics, in which multiple coordination sites make it easy to coordinate to metal ions forming the coordination compounds with novel structures and excellent performance. It is of great significance to investigate the binding properties and mechanism between the coordination compounds with DNA, which could help understanding the mechanism of disease from gene level. The research indicate that that some compounds involved of Cu2+, Co2+ and Ni2+ metal ions exhibit excellent antifungal activity and could possibly interact with DNA. In this thesis, seven title transition metal compounds [Cu0.5L]n (1), [Cu(HL)2Cl2]n (2), [Cu(HL)2Cl2(H2O)] (3), [Cu(L)2(H2O)]n (4), [Cu(L)(phen)(HCO2)]n (5),{[Ni(L)2·(H2O)2]-(H2O)2}n (6) and {[Co(L)2-(H2O)2]-(H2O)2}n (7) have been hydrothermally synthesized by 4-(1H-1,2,4-trizol-1-ylmethyl) benzoic acid (HL) with Cu2+, Co2+ and Ni2+.Structural analyses reveal that compounds 1,2 and 5, Cu(Ⅱ) is bridged through HL, Cl", and formicacid, respectively, featuring 1D chain-structure. In compounds 3,6 and 7, the structural units or the 1D chain units are assembled through hydrogen-bonding into 3D supramolecular framework. In compound 4, 1D chain units-Cu-O-Cu-O-are ligand-bridged into a 3D network. The antifungal activity of the compounds has been explored by the mycelium growth rate method. The measurements show that all the compounds have high antifungal effect on fungi, especially compound 1. Altough the isomers of compounds 6 and 7 are structurally similar to compound 1, both of compounds 6 and 7 with good antifungal activity are not better as 1. The binding properties between the compounds (1,6 and 7) with DNA are studied in methods of gel electrophoresis and ultraviolet spectra. The measurements shows that compounds 1,6 and 7 have good DNA cleavage activity and the order of their intercalative binding strength with DNA is 1>7≥6.