| The pollution problem of phenolic environmental estrogen has become a global problem, causing the attention of the countries. It can influence organisms’normal hor-monal function. Even under the condition of very low concentrations, it can also affect the body’s normal hormone function which leads the body to be teratogenic, carcinogenic, mutagenic, and influence the reproductive system. In recent years, the reports about the food, food packaging materials, pesticides, baby bottles containing phenolic environmen-tal estrogen frequently happen. Therefore, it is particularly important to develop a method that can rapidly, accurately and efficiently detect phenolic environmental estrogens Here, we has established several microminiature electrochemical sensors with the good selectiv-ity, high sensitivity, speed and convenience, which were applied for real samples analysis of phenolic environmental estrogens. The paper contains the following contents:In chapter one, we provides an overview of the characteristics of environmental es-trogens, sources and harm, etc., focusing on the existing treating and detection methods for phenolic environmental estrogens. The content of this thesis was provided.In chapter two, for fast analysis ofpentachbrophenol pollutant, a novel graphite/poly (methyl methacrylate) (PMMA) composite electrode has been fabricated using the viscous prepolymerized MMA solution as an adhesive mixing with graphite. Results indicated that the composite electrode exhibited good electrochemical reversibility, stability, reproduci-bility. Owing to the mesh structure of graphite/PMMA composite, the electrochemical re-sponse of pentachlorophenol at the composite electrode was 2.8 times higher than the conventional carbon paste electrode (CPE). By using the differential pulse voltammetry method, a calibration curve in the concentration range of 1.0x10"7 mol/L～1.0÷10-5mol/L was established for pentachbrophenol, with a detection limit(S/N=3) of 4.6×10-9 mol/L. The recoveries reach 86% to 110% when using the method to determine the 1 water sam-ples. This research provides a fast, simple and sensitive detection tool for envirnmental analysis.In chapter three, based on multi-walled carbon nanotubes-ionic liquid gel soft mate-rial modified screen printing electrode, a new type of bisphenol A electrochemical sensor was designed. The transmission electron microscopy, Raman spectroscopy and electro-chemical methods were used to characterize the soft gel material. The modified electrode exhibits excellent electrochemical response to the bisphenol A. The detection conditions of the pentachlorophenol at the composite electrode were optimized. The concentration linear range for analysis of bisphenol A is between 9.0×10-8 mol/L and 1.0×10"5 mol/L, with a low detection limit of 3.7×10-8 mol/L. The sensor was applied to the detection of environmental samples, and the recovery was 79% to 105%. It is promising in the on-site detection of environmental pollutants.In chapter four, a portable electrochemical instrument for bisphenol A was designed and fabricated, by integrating screen printing electrode modified by multi-walled carbon nanotubes-ionic liquid gel soft material with micro-controller technology. We focus on the design of the hardware system framework of portable detector. Functions of master chip microprocessor and operation guide for the portable instrument are briefly discussed and explained. This portable electrochemical device is suggested to be low-cost, sensitive and simple, which will fulfill the requirement of bisphenol A analysis. |
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