| In recent years, with the improvement of people’s living standards and the increasing popularity of interior decoration, the amount of interior decoration materials is gradually increasing, resulting in a substantial increase in the rate of excessive formaldehyde in indoor air. Formaldehyde is one of the major pollutants in indoor air, which can irritate the human respiratory system and mucous membranes,if the concentration of formaldehyde exceeded the national standard. Excessive formaldehyde can cause skin irritation, coughing, nausea, headache, insomnia and other symptoms. High concentration of formaldehyde has been indicated to induce gene mutations and cancer. Thus, it is a serious impact on people’s health.. Formaldehyde has been LARC as a human carcinogen. Currently, the detection of indoor formaldehyde content is caused more and more people’s attention. Many newly renovated houses, kindergartens, schools etc. will be detected formaldehyde content and confirmed the formaldehyde content is not excessive, before check-in. Therefore, it is very important to find a strong applicability, high sensitivity and high accuracy of method for determination of formaldehyde content in indoor air.The purpose of this article is to find a strong applicability, high sensitivity and high accuracy of method for determination of formaldehyde content in indoor air. People can more accurately judge the quality of indoor air, and take measures or control excessive formaldehyde in time to reduce the harm for human body.We studied the high performance liquid chromatograph and combined with the indoor air quality standards of the People’s Republic of China, then, check and optimize HPLC method for determination of the formaldehyde content in indoor air. The results are as follow:(1). Chromatographic conditions and the mobile phaseThe following high performance liquid chromatography(HPLC)analyses with the chromatographic conditions of Kromasil 100- 5C18 column(4. 6 mm × 250 mm × 5μm); the mobile phase of methanol- acetonitrile – water(70 : 10 : 18 V / V / V),the flow rate of 1. 0 m L / min;the detection wavelength of 360nm;the column temperature of RT.(2). Select the derived condition:PH value of 2, 3, 4, 5(by pH meter) buffer solution was investigated, respectively. According to the results,we determined to select PH value of the buffer 3. We studied the influence of reaction time on the determination of 30, 60, 90, 120 min, according to the results to determine the reaction time was 60 min.(3). Interference testRetention time of raw formaldehyde, 2, 4- 2,4 dinitrophenylhydrazine and product – Dinitrophenylhydrozones is different. So, it’s separated well from the test substance and did not interfere with the determination of formaldehyde, in the air.(4). The standard curve and the detection limitDetection of formaldehyde concentration in 0.02μg / m L-0.40μg / m L range a good linear relationship.(5). Stability TestWe tested the formaldehyde content in the sample after put it in 0h, lh, 6h, 12 h, 24 h, 48 h, 72 h. The results indicate that samples derived after stable at room temperature for 72 h, no significant effect on the results.(6). Recovery testAdd 3 different concentrations of standard solution 10μL and inject six times. Testing the average of six times and measured high recovery rate.(7). Precision and accuracy testTake four different concentrations of samples continuously measured 6 times averaged, high-precision measurements; take five different concentrations of samples, measurement accuracy is good. In conclusionIn conclusion, the method of determination of indoor formaldehyde content by HPLC was rapid, reliable and well reducible. It could be used as a reliable method for measurement of formaldehyde content in indoor air. |
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