Study On Optimization Of Fermentation And Extraction Conditions And Antioxidant Activity Of Polysaccharide From

Ganoderma tsugae polysaccharide is a kind of very important active polysaccharide, which has various medicinal activity and health function that antioxidant, improve immunity, inhibit tumor growth, hypoglycemic, hypolipidemic etc. This thesis with Ganoderma tsugae Murril as raw material, study on extraction,separation and purification of polysaccharide, with the fixed nutritional conditions of liquid fermentation medium and fermentation conditions. And through the in vitro antioxidant experiment of Ganoderma tsugae polysaccharide, Obtained the liquid fermentation conditions of Ganoderma tsugae Murril and the extraction conditions of polysaccharides. The main results are as follows:1. Cultivation of Ganoderma tsugae Murril by liquid fermentation, to find the best single factor: carbon source, nitrogen source and inorganic salt. The best combination of liquid culture medium of Ganoderma tsugae Murril can be found through the orthogonal experiment: Corn flour 5%, wheat bran 0.3%, KH2PO4 0.05%,Mg SO4 0.05%. In the optimal nutrition conditions, shake flask, objective to optimize culture conditions of the polysaccharide yield of fermentation liquid and biomass: the optimum initial p H5.5-6; liquid volume is per 250 ml triangle bottle with 80 ml culture medium; fermentation time is 6d; flask speed is 150r·min-1; inoculation and vaccination age are 10% inoculation quantity and 6d age of inoculation fermentation.2. Study on the extraction process of Ganoderma tsugae Murril mycelium polysaccharide by water extract- alcohol extraction method to find the best extraction temperature 100℃, extraction of 2h and solid-liquid ratio 1:30. Though the orthogonal test to know that different extraction conditions of Ganoderma lucidum mycelia produce different extraction rate, the results show that the degree of influence are temperature,Solid-liquid ratio and extraction times, time.3. Objective to purify refined polysaccharide from Ganoderma tsugae polysaccharide. First of all, use the method of Sevage for polysaccharide deproteinizing, then decolorization of polysaccharides by D101 macroporous resin, at last Chromatography on Cellulose DE-52 cellulose column on the crude polysaccharides, finally, get four components: GT-1、GT-2、GT-3、GT-4. The studieshows that the elution peaks of GT-1 and GT-2 have the better symmetry. It means thant GT-1 and GT-2 are relatively pure polysaccharides.4. In this article, it shows that Ganoderma tsugae polysaccharide have reducing power which increase with the increased concentration of sugar, through reducing capacity, scavenging DPPH· and ·OH free radical, removing superoxide anion and other research on Ganoderma tsugae polysaccharide crude product. This four researchs show that Ganoderma tsugae polysaccharide can remove part of DPPH· free radical, meanwhile there is very obvious effect on scavenging superoxide anion,but ·OH free radical scavenging ability is lower than Vc. The final conclusion is that reducing power research found that the scavenging ability of intracellular polysaccharide is higher than that of extracellular polysaccharide, however the other three studies get the opposite results.