| Phoenix tree seed oil content is high, good quality oil, the phoenix tree seed oil contains large amounts of unsaturated fatty acids on the human body has an important physiological functions and nutritional value. This article without destroying Phoenix tree seed protein, on the basis of using water leaching method and enzymatic improve the Phoenix tree seed oil spiral, and the composition of fatty acid in Phoenix tree seed oil chromatographic analysis, for the Phoenix tree seed oil provide theoretical basis for the research and extension to eat.This experiment leaching method to extract the first investigation hexane, ether, acetone and ethyl acetate solvent for the Phoenix tree seed oil yield efficiency and the effect of determine the optimal extracting solvent ether. And then to feed liquid ratio(1:10, 1:12 and 1:14, 1:16 and 1:18), extraction temperature(45 ℃, 50℃ and 55℃, 60℃, 65℃) and extraction time for(4h, 6h, 8h, 10 h, 12h) three factors in the single factor experiment. On the basis of single factor experiment, fixed solvent ether, to liquid ratio, temperature and time as the independent variable, wutong son oil extraction rate as the response value, three factors five levels of response surface analysis test, a total of 17 sites.On the basis of single factor experiment, fixed solvent ether, to liquid ratio, temperature and time as the independent variable, Phoenix tree seed oil extraction rate as the response value, three factors five levels of response surface analysis experiment. According to the response surface model equation, to determine the best level and combination of various factors. Through the analysis of the mathematical model for the solution of inverse matrix, obtained the optimum technological conditions for temperature 56.8℃, the extracting time 8.5h, material liquid ratio 1:16, two predictive value of 39.25%. To test analysis method of reliability, three verification test, the actual measured yield efficiency of 39.11%, theoretical prediction relative error of 0.36%, respectively.Water enzymatic oil extraction by the Phoenix tree seed oil yield efficiency as the evaluation standard, main pectinase and water and solid-liquid ratio, time, enzyme solution temperature on the enzyme solution Phoenix tree seed oil yield efficiency of the influence of single factor experiment, the experimental results show that the liquid ratio of 1:5, enzymolysis temperature of 55 ℃, enzymolysis time of 6 h extraction conditions, the extraction effect is best, yield efficiency of 39.77%.Finally using gas chromatography to organic solvent extraction method to extract the the Phoenix tree seed oil and enzymatic extraction of the the Phoenix tree seed oil are measured and analyzed the two kinds of method to extract the fatty acids in the Phoenix tree seed oil composition, using external standard method quantitatively analysis and comparison of the fatty acid content in the samples. Found by experiment, two kinds of methods to extract the the Phoenix tree seed oil on the type and content of the fatty acids have certain difference. Water in enzymatic extracts C16:1, C18:1, C18:3(9, 12, 15), C20:5 four kinds of unsaturated fatty acid, and the method of ethyl ether extract of C18:1, C18:3(9, 12, 15), C20:5 three kinds of unsaturated fatty acid. Water enzymatic than ether method a C16:1. In addition, the quantitative analysis shows that the unsaturated fatty acid using C18:1, C18:3(9, 12, 15), C20:5, on the content of the former than the latter in turn were 11.4%, 2.546%, 2.2%. |
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