Screening And Identification Of Environmental Deodorant Microorganisms And Their Application

There are many adverse effects of air pollution on human health. Especially in poorly ventilated indoor environment, air pollution continues to pose a significant threat to health because of harmful decoration materials. Polluting gases, such as formaldehyde and ammonia, their unpleasant odor poses a significant threat to people’s health. Polluting gases can lead to acute and chronic poisoning, cause diseases, and even induce cancer. Pollution in the barn, pastures and market is harmful to the health of livestock and livestock production. Microbial degradation of air pollutant does not require extreme environments or large amounts of nutrients, and will not cause secondary pollution. Management and application of microbial degradation is very convenient. So microbial degradation has very good application in environmental governance. Mainly means and result of research as follow:1. We isolated15strains bacteria and7strains fungi from the soil near the outfall of a chemical plant and the dunghill of a livestock farm.4strains bacteria and4strains fungi have ability of formaldehyde-resistanced, they are HUEB-1, HUEB-5, HUEB-14, HUEB-15and HUEB-3, HUEB-4, HUEB-7, HUEB-9, HUEB-12;5strains bacteria and3strains fungi have ability of ammonia-resistanced, they are HUEF-2, HUEF-4, HUEF-5, HUEF-7and HUEF-1, HUEF-3, HUEF-6. By observation of morphology, physiological and biochemical tests and identification of molecular biology, the results show that strain HUEB-3is Bacillus subtilis, strain HUEB-5is Pseudomonas amygdali, strain HUEB-12is Alcaligenes faecalis, strains HUEF-1and HUEF-5are Penicillium oxalicum, HUEB-4is Byssochlamysfulva.2. HUEF-4and HUEF-5were mixed-cultivated, the results of this experiment showed that the removal rate of formaldehyde is significantly increased, and the best volume ratio of inoculation amount is2:8. HUEF-3and HUEF-12were mixed-cultivated, the results of this experiment showed that the removal rate of ammonia is significantly increased, and the best volume ratio of inoculation amount is6:4.3. Single-factor test analysis, Plackett-Burman design and response surface analysis showed that the best medium components and conditions for removal rate of formaldehyde of the strain HUEF-4and HUEF-5are NaNO32.05g/L, MgSO40.306g/L, KH2PO40.5g/L, FeSO4·7H2O0.04g/L, KCl0.503g/L, CaCl22g/L, glucose lOg/L, yeast extract1.5g/L, initial pH6.0,28℃, in this optimization of the media the removal rate of formaldehyde was increased to83.43±2.2%, and increased25.6%compared to the before optimized.4. Single-factor test analysis, Plackett-Burman design and response surface analysis showed that the best medium components and conditions for removal rate of ammonia of the strain HUEB-3and HUEB-12are KH2PO40.5g/L,(NH4)2SO40.494g/L, MgSO40.3g/L, FeSO4·7H2O0.039g/L, ZnSO40.05g/L, NaCl lg/L, glucose5g/L, peptone1.5g/L, initial pH7.0,34℃, in this optimization of the media the removal rate of formaldehyde was increased to75.1±2.7%, and increased23.9%compared to the before optimized.We isolated some strains bacteria and strains fungi and tested their ability of formaldehyde-resistanced and ammonia-resistanced. Species identification of dominant strains was carried out, and the best medium components and conditions were determined. The results established a basis for further study about principle of decontamination of strains and research on the application.