Synthesis, Properties And Bio - Application Of Fluorescence Probes Based On Nucleotides And Nitro Reductase Assays

Fluorescent probe is a tool that can transfer the molecular recognition events into optical signals. Because of the high sensitivity, specificity, and simplicity of implementation and so on. Fluorescent probe have attracted the attention of the researchers in recent years, and it have been widely used in chemical and biological science, clinical diagnostics, materials science, and environmental chemistry. In this paper, fluorescent probes for nucleotides and nitroreductase have been constructed using1,8-Naphthalimide and benzopyran nitrile as fluorophores respectively. The fluorescent probes are based Intermolecular reaction and Hydrogen bonding. The concrete contents are as follows:A series of1,8-naphthalimide-based analogs were developed. In DMSO, compound1had been proved to be an effective and selective colorimetric and fluorescent sensor for recognition of GMP, TMP, and UMP over other structurally similar nucleotides. Among all the tested nucleotides, only addition of GMP, TMP, and UMP resulted in a color change of fluorescence from blue to brown with a fluorescence enhancement of more than600-fold, and the colorless solution turned brown. It was demonstrated that1formed multi-hydrogen bonds with the related base groups in the nucleotide, which was supported by NMR spectroscopic titration, theoretical calculations, and spectral tests performed using various solvent compositions.1was successfully applied to fluorescence imaging for GMP, TMP, and UMP in C. elegans, demonstrating its utility as a fluorescent chemosensor for detecting GMP, TMP, and UMP in in vivo imaging.A new benzopyran nitrile-based fluorescence probe (Al) was successfully designed and synthesized for the excellent selective sensing of NTR, nitrobenzene as specific center. After added NADH and NTR in PBS Buffer(0.01mol/L, pH=7.4,1%DMSO), nitro was selectively and rapidly reduced to amine, and a color change of fluorescence from the light red to yellow, Remarkable enhancement in fluorescence intensity. A1was successfully applied to fluorescence imaging for NTR in C. elegans, imaging C. elegans imaging revealed that the probe is lower toxicity, good water solubility and biological compatible, which could applied to In vivo imaging.