| Processing of seafood produces large amounts of by-products, which would be discarded,causing great waste of resources and environmental pollution. The main research contents and results were as follows:A sulfated polysaccharide(AGSP) was extracted from gonads of pacific abalone by alkali hydrolysis. The osteogenic activity induced by BMP-2 of AGSP was investigated using C2C12 myoblast cells by measuring alkaline phosphatase activity. The monosaccharide and disaccharides composition were analyzed PMP derivation followed by HPLC-PAD-MSn analysis. AGSP enhanced ALP activity induced by BMP-2 in a dose dependent manner at concentrations ≥10 μg/ml, but its activity was the weakest among the three polysaccharides corresponding to its lowest sulfate content. Man, Gal, Glc A, Xyl, Fuc and Rha were found in AGSP. Glc N and Gal N were not observable, so it illustrated that AGSP was the non-glycosaminoglycan polysaccharide.The polysaccharides(1.4-AGP, 3-AGP and MAGP) isolated from abalone gonad by double enzyme enzymatic hydrolysis were separated as three fractions: two fractions eluted by 1.4 mol/L and 3 mol/L Na Cl, respectively, and the fraction of the mixed supernatants after alcohol precipitation of the two eluents. The analysis results illustrated that the protein contents of three polysaccharides were low, less than 5%, and their neutral sugar contents were 18±3%. The 3-AGP had the largest contents of sulfated polysaccharide and sulfate group, 60.22% and 7.77%, respectively. Acid hydrolysates of the polysaccharides were analyzed by PMP-HPLC-MS. The results indicated that 1.4-AGP and 3-AGP had similar composition of monosaccharides, containing the main components of Xyl, Gal, Man, Fuc, Ara,and Glc UA, whereas others, e.g. Glc N, Gal N and Rib, were in a minor content. However, Rha was only found in the MAGP, while the other monosaccharide composition was similar to the other two kinds of polysaccharides. In addition, two kinds of disaccharides both exsit in all of the three polysaccharides, and only differs in the relative contents.Polysaccharides(SVP) were prepared from viscus of Patinopecten yessoensis by double enzyme enzymatic hydrolysis. Their structure features were analyzed by chemical methods,PMP derivation followed by HPLC-MS analysis and infrared spectrum. The contents of neutral sugar, protein, sulfate, sulfated polysaccharides were determined as 75.37%,4.09%,0.63% and 8.77%, respectively. IR spectrum showed characteristic absorption of polysaccharides. Acid hydrolysates of the polysaccharides were analyzed by PMP-HPLC-PDA-MS. The results indicated that Xyl, Gal, Man, Rha, Fuc and Ara were the main components, whereas others,e.g. Glc N,Gal N,Gal A and Glc,were in a minor content.In addtition, Glc A-(1→3)-Gal N, Glc A-(1→2)-Man and two polysaccharides composed of a hexose and a hexuronic acid were found in viscus of Patinopecten yessoensis by analyzing the resulting disaccharides.The polysaccharides(SBLP) were extracted from the boiled liquid of sea cucumber by double enzyme enzymatic hydrolysis, further enzymatic hydrolysis and hydrogen peroxide treatment. The monosaccharide and disaccharides composition were analyzed PMP derivation followed by HPLC-MS analysis. Glc N, Gal N, Gal, Man, Rib were the main monosaccharide components of the polysaccharide from the boiled liquid of sea cucumber, whereas others, e.g.Xyl, Fuc, were in a minor content. In addtition, Glc A-(1→3)-Gal N was found by analyzing the resulting disaccharides, which inferred that chondroitin sulfate was in the boiled liquid of sea cucumber.This research provides the information of polysaccharides in the processing by-products of abalone, sea cucumber, scallop and offers the scientific basis for the development and utilization of these by-products. |
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