Galaxolide And Cadmium Compound Contaminated Soil With Plant- Microbial Combined Remediation Research

Galoxolide(HHCB) is a commonly used raw materials for making cosmetics, washing products, perfume, skin care products and so on. And with the daily life using, it first enters the domestic sewage, and through sewage irrigation, sludge agricultural application and so on a variety of ways to continuously enter the soil. And cadmium(Cd) is a kind of common pollution element at heavy metal contaminated soil. HHCB and Cd compound pollution can be enhanced single polluted toxicity for animals and plants, therefore, has become a new type of compound pollutant with potential hazard in the environment. This paper use of heavy metals Cd enrichment plant purple jasmine and maidenhair with HHCB degrading bacteria, for HHCB and Cd combined contaminated soil conduct plant- microbes combined Remediation, study the feasibility of their repair. And by denaturing gradient gel electrophoresis(DGGE) to further analyze the molecular mechanism of dynamic changes at the micro level, The main conclusions of this paper are as follows:1. Using flame atomic absorption spectrometry and gas chromatography mass spectrometry for pot experiment harvested in plant and soil samples Cd and HHCB conduct test. The results show that maidenhair is better than purple jasmine the enrichment effect of the Cd, and two kinds of the plants aboveground parts for better enrichment effect of Cd, bacteria solution can promote the enrichment of cadmium in plants. The presence toxicity of HHCB, it inhibited the enrichment of Cd in plants. HHCB higher the concentration, lower the degrading bacteria the degradation efficiency. And whether or not adding degrading bacteria, plant maidenhair soil the degradation rate slightly higher than the soil planting purple jasmine. In addition,when the content of Cd is 0mg/kg the degradation rate of HHCB was higher than the content of Cd is 5mg/kg degradation rate of HHCB. The experiment proved that the use of maidenhair and HHCB degrading bacteria combined remediation of HHCB and Cd contaminated soil is a feasible technique.2. Using PCR-DGGE technique testing diversity of microbial communities change in the soil HHCB and Cd combined pollution, and using Quantity One software DGGE gel electrophoresis profiles of a preliminary analysis. The results showed that when HHCB and Cd contents were 0mg/kg in the soil planting purple jasmine, its soil bacterial population diversity index up to 3.066; When HHCB and Cd contents were 0mg/kg in the soil planting maidenhair, its soil bacterial population diversity index up to 3.125. The diversity index of bacterial populations in HHCB and Cd composite Pollution was lower than HHCB or Cd single contaminated bacterial population diversity index. HHCB and Cd complex pollution bacterial population diversity index of up to 3.012. HHCB single pollution bacterial population diversity index up to 3.067. Cd single pollution bacterial population diversity index up to 3.054. Cd single pollution bacterial population diversity index compared with HHCB single pollution bacterial population diversity index. Cd single contaminated bacterial population diversity index were lower than when HHCB content of 100mg/kg the bacterial population diversity index, and it were higher than when HHCB content of 300mg/kg the bacterial population diversity index. Experiments show that the HHCB and Cd Combined Pollution of soil will certain impact on the soil bacterial population diversity.