The Study On Anti-aging Of Seleno-β-lg

Seleno-β-lg was regarded as the research object in this experiment. Initially, its safety dose was confirmed after the acute and subacute toxicity testing had been investigated. Otherwise, the anti-aging mechanism of Seleno-β-lg was explored by ethology, biochemistry, tissue staining and Real-time PCR in D-galactose mice from free radical theory and Neuroendocrine doctrine theory.Firstly, toxicological safety of the Seleno-β-lg on mice was studied and the 50% lethal dose (LD50) was 15.94 mgSe/kg, which calculated by improved Kou method.Secondly, the anti-aging activity of Seleno-α-lg was researched in the mice aging model induced by D-galactose. The learning and memory ability were observed by morris water maze test, results showed that in Seleno-β-lg groups, the escape latency was shortened, numbers of crossing the platform were reduced and the percentage of swmming distance in the quondam platform qurdrant were evidently higher than in the model group mice. These data indicated that Seleno-β-lg could postpone the decay of learning and memory ability to some extent. After behavioral test, the mice were executed and various biochemical indices were assayed. The results showed that acetylcholinesterase (AchE) activity in the brain tissue and serum was significantly decreased by Seleno-β-lg, it could maintain the stability of the cholinergic system which could postpone the aging of mice. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were increased significantly and malondialdehyde (MDA) was decreased in different issues after the intake of Seleno-β-lg. Cerebral cortex was observed by HE staining, Seleno-β-lg could slow down the reduction and reshape of neure to some extent, as a result, Seleno-β-lg could protect the cerebral cortex from damaging.Finally, mRNA was extracted in brain and liver and the results of Real-time PCR showed that the expression of GPX1、GPX3、GPX4 in middle dose group was significantly increased than that in model group. In summary, Seleno-β-lg had a good effect of anti-aging, it could significantly postpone the aging progress of aging mice induced by D-gal.