The Screening Of Halophiles And Salt-Tolerant Phenol Degrading Bacteria In Badain Jaran Desert Salt Lake And Characteristics Degradation Of Phenol

High salinity wastewater that contains phenol what is discharged from coal chemical industry produces great harm to organism. Changes in salinity in this wastewater inhibit microbial degradation of phenol. It allows researchers to screen out microorganism that can survive in high salinity environment contains phenol very urgent. In this study, two halophilic phenol degradation strains H17 and CL are screened what can use phenol as carbon source. They also are identified by 16S rRNA gene. By a batch culture method, we research strain H17 and CL phenol degradation characteristics in growth conditions under different culture conditions. We also use cell immobilization techniques and mixed bacteria to increase the degradation effect. Then we research the bacteria growth and phenol degradation characteristics. The main findings are as follows:(1) Two halophilic phenol degradation strains H17 and CL are screened from the Badain Jaran Desert salt lake sediments throw using enriched screening method. From the 16S rRNA gene analysis, strains H17 has the highest similarity with Halomonas taeanensis BH539, so the strain is named Halomonas taeanensis H17; strains CL has the highest similarity with Staphylococcus caprae ATCC 35538, so the strain is named Staphylococcus caprae CL.(2) By a batch culture method, strain H17 and CL growth and phenol degradation characteristics are studied, when strains H17 and CL survive in phenol concentration 200 mg/L, NaCl concentration 10%, pH 8, temperature 30℃, the appropriate amount of additional carbon and organic compound nitrogen source. The degradation rate of phenol and strain its own biomass reaches max, the biomass (OD6oo) is 1.8 and 1.6, and the degradation of phenol is 88% and 83%.(3) By a microorganism immobilization technology, strain H17 and CL growth and phenol degradation characteristics are researched in in high salinity and phenol solution. At a phenol concentration of 800 mg/L, the degradation rate improves espectively 38% and 17%. When H17:CL is 3:7, the degradation rate of phenol is more than 70%. The degradation rate is about 69% at a phenol concentration of 600 mg/L and more than 57% at a phenol concentration of 1500 mg/L under this condition. It shows mixed bacteria and immobilized microorganism technology can greatly increase the concentration of phenol strain tolerance, and the efficiency of the degradation of phenol.(4) By comparing strain H17 and CL, strain H17 belongs to Halophilesis and is suitable for degrading high salt concentration, a wide range of pH, low carbon high salt wastewater contains phenol. Strain CL belongs to tolerant bacteria and is suitable for high phenol concentration, a wide range of temperature high salt wastewater contains phenol.