Study On Selection Of Heterotrophic Nitrobacyeria And Denitrification Of Landfill Leachate

Domestic daily life produces the vast majority of domestic waste landfill processes are used for processing,The landfill leachate will produce secondary pollutants. Landfill leachate containing high concentrations of organic matter, organic nitrogen and ammonia, whereas at high concentrations of organic leachate biological nitrogen removal is a problem of governance.From traditional biological nitrogen removal by the nitrification and denitrification of denitrifying bacteria in two roles is complete, but the high concentration organic wastewater can inhibit the growth of bacteria autotrophic nitrification and denitrification capacity, removal is not ideal. In this paper, the use of heterotrophic nitrification bacteria at high concentrations of organic nitrogen conditions still has a strong starting performance,From a landfill leachate treatment plant pilot processing apparatus biofilm and bacterial sludge can have screened at a high concentration of organic nitrogen and carbon removal capacity and efficient condition heterotrophic nitrification bacteria,Combined with Immobilized leachate treatment. Conclusions show:1, Enrichment culture leachate treatment apparatus biofilm bacteria mud, pick out the 29 colonies, after several separation and purification to give pure four strains, and then screened strains in pure culture ability in addition to carbon and nitrogen growth ability to detect, and ultimately selected two nitrogen and carbon removal effect and rapid growth of the strain, designated as strain S1 and strain S3.2, Detection screening nitrification and denitrification capacity and capacity utilization nitrite strain S1 and strain S3, and strain S1 and strain S3 of morphological observation, the use of PCR molecular biology techniques species identification, identification results:strain S1 is Alcaligenes faecalis (Alcaligenes faecalis strain NBRC 13111), strain S3 is Acinetobacter(Acinetobacter pittii strain ATCC 19004), had strong nitrification and denitrification.3, With 2 strains are screened by inoculum size 1:1 complex composed of bacteria, domestication actual landfill leachate,When domesticated complex bacteria stable, garbage leachate treatment small pilot test,In addition to changes in carbon and nitrogen and investigated the ability at different pH, temperature, DO conditions.The results showed that the composite bacteria treated leachate optimum pH value is 7.0 to 8.0, optimum temperature conditions for 25℃, the best DO 1mg/L. The composite bacteria garbage leachate treatment of ammonia nitrogen removal rate was up 58.3%, total nitrogen removal rate was 47.1%, COD removal rate was 79.6%, showing a composite strain garbage leachate has good removal.4, Combined with the actual strain of the composite effect of landfill leachate treatment,Charcoal adsorption method using immobilized microbial strains were fixed,Establish the running time, the amount of the microbial support, COD influent concentration of three factors and three levels orthogonal experimental program. Upgrading and optimization of complex strain on landfill leachate treatment effect of nitrogen in addition to carbon.Contrast bacteria treatment of landfill leachate, landfill leachate treatment by immobilized microorganisms on removal significantly enhanced.Orthogonal experiment results show that when the running time of 48h, microbial support amount is 20g, the COD influent concentration of 1031mg/L, the immobilized microbial denitrification reactor has the best ability in addition to carbon, ammonia removal efficiency was 83.1% total nitrogen removal rate was 61.1%, COD removal rate was 83.6%.