| Aniline aerofloat is a common floatation wastewater which brings more difficult to the treatment of Ore dressing wastewater with hard degradable for toxic and harmful. As a high-rate anaerobic reactor, Anaerobic Baffled Reactor has excellent ability to remove organic pollutants. An ABR reactor was set up and sludge domestication inoculated anaerobic tank sewage treatment plant sludge, the sludge concentration was adjusted to 21560 mg/L. Hydraulic Retention Time was 24 h, a steady degradation of aniline aerofloat and COD removal situation with the operation of HRT、pH and other experimental factors external carbon ratio, and studies the properties and granular sludge formation mechanism. High-throughput sequencing technology was used to analysis community structure within the stable operation of the reactor microbial. The main conclusions are as follows:(1)A fixed HRT and increased volume loading was used to startup ABR. Gradient sludge acclimation nigrosine by increasing aniline aerofloat concentration. After 100 day’s running, domestication stable, the influent carbon source aniline aerofloat reached 250 mg/L, the degradation rate was 70.00% and remained stable, the lowest concentration of the effluent was 72.95 mg/L, the degradation efficiency reached 70.22%. The reactor was started successfully, the frontend compartments played major roles on removal of organic. The effluent VFA content was 200 mg/L or less, pH was 7.0, the system was not acidification.(2)HRT, pH, and external carbon degradation had great impacts on removal of aniline aerofloat in ABR. The best conditions was HRT=24 h and pH=7.0. The efficiency of COD removal reached 74.77%, aniline aerofloat degradation rate was 75.20%. When aniline aerofloat to glucose at the ratio of 1:1, the reactor effluent can achieved the best result. The efficiency of COD removal reached up to 80.51%, the highest rate of degradation of aniline aerofloat was 77.15%.(3)A large number of granular sludge was in ABR, which diameters were about 0.8～4.5mm, black or gray-black, spherical or ellipsoidal, smoothly. The highest level of aerobic granular sludge ratio was the first compartment, reaching 85%. Over compartment, particles size showed a decreasing trend. Microbial community structure was formed in ABR which could adapt to aniline aerofloat and degradation of aniline aerofloat.(4)Large amounts of Filamentous Bacteria, Staphylococcus Aureus and Bacillus were presence in granular sludge, Filamentous Bacteria and Bacillus connected the cores to form small particles of sludge. Small particles grow into large particles, parcel of filamentous bacteria and bacillus formed mature granular sludge under the winding.(5)The first and second compartments sludge were made to high-throughput sequencing, harvested effective sequence,35003 and 35632, respectively. Classify optimized operation, the sample coverage of the first and second compartments (Coverage) were 90.52% and 89.68%, respectively. Reasonable sample sequencing was get and it is a true reflection of the reactor microbial community structure situation. Miseq high-throughput sequencing results showed that Chloroflexi accounted for most of sequence (24.73% and 33.60%), followed by Firmicutes (24.17% and 20.92%) and Proteobacteria (18.93% and 20.88%) at the phylum levels. At the genus levels, Caldisericum, Leptolinea, Leuconostoc and Bacillus were identified, which were considered positive to the degradation of aniline aerofloat. |
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