Study On Purification And Purity Identification Of Nicandra Polysaccharides

Nicandra Physaloides is a kind of solanaceae plant of Chinese herbal medicine, some areas in the south of China, it can prevent and treat various diseases, has the very high pharmacological properties. It contains oleic acid, linoleic acid, alkaloids, brass, fats and proteins, and other ingredients. In addition, Nicandra physaloides also contains a lot of polysaccharide compounds. At present domestic and foreign is craving in studying each kind of plant the polysaccharide compound, in order to improve the efficiency of industrial production of Nicandra physaloides.In this paper, the extraction, purification and purity detemination of nicandra polysaccharides were studied.Orthogonal experiments L9(34) was designed on the basis of the single factor. Determining the method of extraction of polysaccharide from nicandra physaloides: the enzymatic concentration was 3%, the extraction pH was 7.5, the extraction temperature was 55 ℃, and the extraction time was 50 min. The extraction rate of polysaccharide was 6.02%.Determining the method of deproteinization from polysaccharide: enzymatic extraction condition and tow joint Sevage method, the deproteinization rate reached to 77.33%.Orthogonal experiments L9(33) was designed on the basis of the single factor. Determining the method of decolorization from polysaccharide: the resin concentration was 3%, the extraction temperature was 40 ℃, and the extraction time was 2h. The decolorization rate of polysaccharide was 70.2%.Through the DEAE-cellulose column chromatography- classification, determine PⅠ、PⅡ、PⅢ、P Ⅴis the main component of polysaccharide; on Sephadex G-200 column chromatography, the single peak symmetry can be obtained; Freeze-thawing method was also used to prove that PⅠ、PⅡ、PⅢ、P is aⅤ n uniform components.