| By enrichment culture, a stable five-member bacterial consortium NC1 was isolated from corn-planted soil. After 32 hours, consortium NC1 that was capable utilizing 100mg/L atrazine to nontoxic cyanuric acid. Based on physiological and biochemical tests and 16 S r DNA gene sequence analysis, five bacterial strains(YJY1-YJY5) were identified Enterobacter sp.; Achromobacter sp.; Pseudomonas aeruginosa; Shewanella sp; Klebsiella sp., respectively. Strain YJY4 and YJY5 capable of utilizing atrazine as a sole nitrogen s ource for growth. Degradation experimental results show that consortium NC1 have favorable atrazine degradation effect.A bacterium strain, YJY4 could utilize atrazine as the sole nitrogen source for growth was isolated from agricultural black soil in Northeast of China. From the different environmental factors affect the degradation rate of growth of strain and degradation of atrazine angle, how to effectively use atrazine atrazine degrading bacteria reduce harm to the environment. Mainly from the PH, different carbon sources, nitrogen sources, and various concentrations of atrazine content rate of growth of atrazine strain to work. Use regularly train every 12 h were measured by the method OD600 nm values, confirmed that the strain degrading bacteria in a w ide range of optimal p H atrazine can be great growth. 16 S rRNA sequencing identified YJY4 as a Shewanella sp. PCR analysis and sequencing confirms the presence that strain YJY4 contained atrazine-degrading genes atzA, atz B, atzC genes. These genes revealed high sequence similarity with those in Pseudomonas sp. ADP and Arthrobacter sp. TC1. The strain YJY4 was capable of degrading the whole atrazine(100 mg/L) to cyanuric acid after 36 h. To our knowledge, strain YJY4 was the first reported Shewanella sp. in pure culture growing with atrazine as a sole source of nitrogen, therefore, strain YJY4 may contribute to efforts on atrazine biodegradation and abundant resource of atrazine degradation strains. |
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