Koumiss Lactobacillus Isolation And Identification Of HS-R9, Bacteriocins Extract And Its Effect On The Immune Barrier In Mice

About Koumiss extracted from Koumiss bacteriocins or Lactobacillus Bovine source of pathogenic E. coli wild reports currently only relates to in vitro antibacterial, antifungal mechanism of pathogenic E. coli wild bovine source body reports and immune barrier function of mice almost no impact. So, Koumiss study from the Xilin Gol League in Inner Mongolia region collected and extracted from Lactobacillus HS-R9, Lactobacillus HS-R9 bacteriocins as a test sample. Oxford Cup method and the use of double dilution method reveals substances on the above sources of wild cattle pathogenic E. coli antibacterial effect.For koumiss and other microbial components extracted from a preliminary analysis by in vitro inhibition test Koumiss explore other microorganisms on six sources of wild cattle pathogenic pathogens antibacterial effect, initially identified the antibacterial spectrum. Antibacterial test inquiry Koumiss other microbial treatment of bovine source wild pathogenic E. coli, in addition to the impact of pathogenic E. coli infection in mice intestinal mucosal immune barrier function to be described. The results showed that:1. Collected from the Xilin Gol League in Inner Mongolia region koumiss can be extracted and purified strains selected after a temporary strain named JJ. Bacterial morphological identification, physiological and biochemical characteristics identification, taxonomic identification 16SrDNA sequence analysis workstation by NCBI GenBank database, Nueleotide BLAST program than the result of the determination of the strain called Lactobacillus M HS-R9, ammonium sulfate extraction method the Lactobacillus HS-R9 bacteriocins.2. By Oxford cup double dilution method and two in vitro antibacterial methods can yield different proportions (1/2,1/4,1/8) sour mare’s milk, Lactobacillus HS-R9, Lactobacillus Bacillus HS-R9 bacteriocins can inhibit the growth of pathogenic E. coli O78, where in 1/2 Koumiss dope, lx Lactobacillus HS-R9 and 1x Lactobacillus bacteriocin antibacterial HS-R9 better antibacterial diameters of 20.11 mm,20.20 mm,19.51 mm, MIC was 0.061mg/mL, 0.028 mg/mL,0.053 mg/mL, MBC was 0.242mg/mL,0.111 mg/mL,0.210 mg/mL. Koumiss liquid,1/2 Lactobacillus HS-R9,1/2 Lactobacillus HS-R9 bacteriocins proportion, followed by antibacterial diameters of 19.61 mm,19.17 mm,18.24 mm, MIC, MBC was 0.029 mg/mL,0.059mg/mL,0.059mg/mL,0.237mg/mL and 0.117mg/mL,0.472mg/mL.3. Acid mare’s milk, Lactobacillus HS-R9, Lactobacillus HS-R9 supernatant liquid fermentation, Lactobacillus HS-R9 bacteriocins contain high protein, respectively 1.750,1.490,1.630,1.237 mg/mL. Determination of total amino acids and 17 amino acids were 1.99,1.262,1.67,0.686mg/100 mg.10 Determination of organic acids and organic acid contents were quite different. They enterohaemorrhagic Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus, Streptococcus agalactiae six kinds of disease-causing pathogens are antibacterial, antifungal diameter distributed between 26-31 mm, respectively,0.027-0.031 mg/mL about changes between 0.219-0.500mg/mL MIC, MBC.4. By mice antimicrobial testing shows that the concentration of pathogenic E. coli O78 when 5.0×1010～7.5×1010CFU/mL,80% mortality in mice, it was 80% MLD. Microplate reader measured obtaining OD values between 1.35 to 1.36.5. Koumiss and Lactobacillus HS-R9 bacterial infection prime source of wild bovine pathogenic E. coli O78 in mice has a protective effect, the protection rate of 66.6%, close to the rate of protection of ciprofloxacin hydrochloride. Koumiss stock solution, pH= plant 2:00 HS-R9 lactobacilli bacteria of the preferred antimicrobial dose mice were 1.5mg/mL,3.0mg/mL.6. Koumiss and Lactobacillus HS-R9 bacteriocins were increased immune organ index mice and monocyte-macrophage phagocytosis and immunity.7. Koumiss and Lactobacillus HS-R9 bacteriocins can be significantly increased IL-2, IL-6, IL-8 concentration in the blood of mice model and IFN-7, and significantly reduced IL-10 and TNF-the concentration of a, significantly enhanced IgA, IgG, IgM levels of.